On-line liquid chromatography coupled with high field NMR and mass spectrometry (LC-NMR-MS): a new technique for drug metabolite structure elucidation

K I Burton; J R Everett; M J Newman; F S Pullen; D S Richards; A G Swanson

doi:10.1016/s0731-7085(96)02034-1

On-line liquid chromatography coupled with high field NMR and mass spectrometry (LC-NMR-MS): a new technique for drug metabolite structure elucidation

J Pharm Biomed Anal. 1997 Aug;15(12):1903-12. doi: 10.1016/s0731-7085(96)02034-1.

Authors

K I Burton¹, J R Everett, M J Newman, F S Pullen, D S Richards, A G Swanson

Affiliation

¹ Pfizer Central Research, Sandwich, Kent, UK.

PMID: 9278896
DOI: 10.1016/s0731-7085(96)02034-1

Abstract

High performance liquid chromatography has been coupled simultaneously to high field NMR and MS detectors, giving UV, NMR and mass spectra for each component in a mixture, after on-line separation. This powerful new tool for the structure elucidation of components in mixtures without isolation has been successfully applied to the analysis of the metabolites of paracetamol in human urine.

MeSH terms

Acetaminophen / chemistry
Acetaminophen / urine*
Analgesics, Non-Narcotic / chemistry
Analgesics, Non-Narcotic / urine*
Chromatography, Liquid*
Humans
Magnetic Resonance Spectroscopy / methods*
Male
Mass Spectrometry / methods*
Molecular Structure
Online Systems*
Reference Values

Substances

Analgesics, Non-Narcotic
Acetaminophen