Abstract
Studies have suggested that phenobarbital (PB) induces members of the CYP1A subfamily by both transcriptional and post-transcriptional mechanisms. Using the Ahr -/- mice, we examined the induction of CYP1A1 and CYP1A2 by PB. CYP1A2 mRNA and protein were induced by PB in the null mice, suggesting that CYP1A2 is regulated by PB by a mechanism independent of the aryl hydrocarbon receptor (AHR). In contrast, the regulation of CYP1A1 is highly dependent on the AHR.
MeSH terms
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Animals
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Cytochrome P-450 CYP1A1 / biosynthesis*
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Cytochrome P-450 CYP1A2 / biosynthesis*
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Enzyme Induction
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Mice
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Mice, Inbred C57BL
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Mice, Inbred Strains
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Mice, Knockout
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Microsomes, Liver / drug effects
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Microsomes, Liver / enzymology*
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Phenobarbital / pharmacology*
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Protein Biosynthesis
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RNA, Messenger / biosynthesis
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Receptors, Aryl Hydrocarbon / deficiency
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Receptors, Aryl Hydrocarbon / physiology*
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Transcription, Genetic
Substances
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RNA, Messenger
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Receptors, Aryl Hydrocarbon
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Cytochrome P-450 CYP1A1
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Cytochrome P-450 CYP1A2
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Phenobarbital