Q172H replacement overcomes effects on the metabolism of cyclophosphamide and efavirenz caused by CYP2B6 variant with Arg262

Drug Metab Dispos. 2011 Nov;39(11):2045-8. doi: 10.1124/dmd.111.039586. Epub 2011 Aug 5.

Abstract

There are a number of reports indicating that CYP2B6*6 (c.516G>T and c.785A>G) is responsible for decreased clearance of efavirenz (EFV), although increased disposition of cyclophosphamide (CPA) in individuals with this polymorphism was observed. Thus, we hypothesized that the effects of the two single nucleotide polymorphisms (SNPs) of CYP2B6*6 on the metabolism of drugs might be considerably different between these two agents. To clarify this possibility, we expressed two major variants of this enzyme, CYP2B6.6 (Q172H and K262R) and CYP2B6.4 (K262R), and investigated metabolic activities of these variants toward EFV and CPA. Kinetic analyses clearly indicated that CYP2B6.4 possessed enhanced metabolic activity toward EFV compared with that of the wild-type enzyme (CYP2B6.1), whereas CPA was metabolized less efficiently by CYP2B6.4 than by CYP2B6.1. On the other hand, CYP2B6.6 showed a completely opposite character, suggesting that Q172H gives inverse effects on metabolic activities of CYP2B6 affected by K262R. Although it is recognized that effects of amino acid change in cytochrome P450 on the metabolic activity depend on substrates, this study revealed SNPs giving an opposite effect on the metabolism of two clinically important drugs currently used. Furthermore, this study provides the first evidence that Q172H can reverse the direction of the effect caused by K262R in CYP2B6 on the metabolism of certain drugs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkynes
  • Animals
  • Aryl Hydrocarbon Hydroxylases / genetics*
  • Aryl Hydrocarbon Hydroxylases / metabolism*
  • Benzoxazines / pharmacokinetics*
  • Cyclophosphamide / pharmacokinetics*
  • Cyclopropanes
  • Cytochrome P-450 CYP2B6
  • Cytochrome P-450 Enzyme System / metabolism
  • Humans
  • Inactivation, Metabolic
  • Liver / enzymology
  • Liver / metabolism
  • Mixed Function Oxygenases / metabolism
  • Oxidoreductases, N-Demethylating / genetics*
  • Oxidoreductases, N-Demethylating / metabolism*
  • Pharmacokinetics
  • Polymorphism, Single Nucleotide
  • Protein Processing, Post-Translational

Substances

  • Alkynes
  • Benzoxazines
  • Cyclopropanes
  • Cyclophosphamide
  • Cytochrome P-450 Enzyme System
  • Mixed Function Oxygenases
  • Aryl Hydrocarbon Hydroxylases
  • CYP2B6 protein, human
  • Cytochrome P-450 CYP2B6
  • Oxidoreductases, N-Demethylating
  • efavirenz