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Transport inhibition of digoxin using several common P-gp expressing cell lines is not necessarily reporting only on inhibitor binding to P-gp.
PLoS One. 2013 Aug 16;8(8):e69394. doi: 10.1371/journal.pone.0069394. eCollection 2013.
PLoS One. 2013.
PMID: 23976943
Free PMC article.
If the KI is defined by the free energy of binding to P-glycoprotein, which kinetic parameters define the IC50 for the Madin-Darby canine kidney II cell line overexpressing human multidrug resistance 1 confluent cell monolayer?
Lumen AA, Acharya P, Polli JW, Ayrton A, Ellens H, Bentz J.
Lumen AA, et al.
Drug Metab Dispos. 2010 Feb;38(2):260-9. doi: 10.1124/dmd.109.029843. Epub 2009 Nov 4.
Drug Metab Dispos. 2010.
PMID: 19889884
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