Metabolism of chlorpyrifos by human cytochrome P450 isoforms and human, mouse, and rat liver microsomes

J Tang, Y Cao, RL Rose, AA Brimfield, D Dai… - Drug metabolism and …, 2001 - ASPET
One of the factors determining the toxicity of chlorpyrifos (CPS), an organophosphorus (OP)
insecticide, is its biotransformation. CPS can be activated by cytochrome P450 (CYP) …

In vitro human metabolism and interactions of repellent N, N-diethyl-m-toluamide

…, RL Rose, JA Goldstein, WG Taylor, AA Brimfield… - Drug metabolism and …, 2002 - ASPET
Oxidative metabolism of the insect repellentN,N-diethyl-m-toluamide (DEET) by pooled
human liver microsomes (HLM), rat liver microsomes (RLM), and mouse liver microsomes (MLM) …

Mouse monoclonal antibodies against paraoxon: potential reagents for immunoassay with constant immunochemical characteristics

AA Brimfield, DE Lenz, C Graham… - Journal of Agricultural …, 1985 - ACS Publications
Mice were immunized with (p-aminophenyl) paraoxon conjugated to protein by diazotization.
The production and isolation of permanent hybridoma cell lines from the splenic …

[PDF][PDF] Quantification of the organophosphorus nerve agent soman by competitive inhibition enzyme immunoassay using monoclonal antibody.

KW Hunter Jr, DE Lenz, AA Brimfield, JA Naylor - FEBS letters, 1982 - core.ac.uk
The organophosphorus compound soman (methylphosphonofluoridic acid 1, 2, 2-trimethylpropyl
ester) is a potent cholinesterase inhibitor [1, 2] and a chemical warfare agent. The rapid …

Development of a monoclonal antibody based enzyme immunoassay method for analysis of maleic hydrazide

RO Harrison, AA Brimfield… - Journal of Agricultural and …, 1989 - ACS Publications
MATERIALS AND METHODS All chemicals were reagent grade or better and were supplied
by Aldrich Chemical Co. unless noted otherwise. MH derivatives were analyzed for purity by …

Development of immunoassays for detection of chemical warfare agents

DE Lenz, AA Brimfield, LA Cook - 1997 - ACS Publications
With the advent of enzyme linked immunoabsorbent assays (ELISA) and monoclonal antibodies,
there has been considerable effort devoted to the development of antibodies to detect …

Measurement of monoclonal antibody concentrations in hybridoma cultures: Comparison of competitive inhibition and antigen capture enzyme immunoassays

JM Bosworth Jr, AA Brimfield, JA Naylor… - Journal of …, 1983 - Elsevier
Competitive inhibition and antigen capture enzyme immunoassays were compared for the
measurement of mouse monoclonal IgG1 antibody produced by a hybridoma culture. Both …

Identification of products arising from the metabolism of cis-and trans-chlordane in rat liver microsomes in vitro: outline of a possible metabolic pathway

AA Brimfield, JC Street, J Futrell, DA Chatfield - Pesticide Biochemistry and …, 1978 - Elsevier
The metabolism of pure cis- and trans-chlordane was studied in vitro. Microsomal preparations
from the livers of male rats induced with cis- or trans-chlordane in feed for 10 days were …

Oxidation of thiodiglycol (2, 2′‐thiobis‐ethanol) by alcohol dehydrogenase: Comparison of human isoenzymes

BF Dudley, AA Brimfield… - Journal of Biochemical …, 2000 - Wiley Online Library
Sulfur mustard is a chemical warfare agent that causes blistering of the skin and damages
the eyes and airway after environmental exposure. We have previously reported that …

Microsomal activation of chlordane isomers to derivatives that irreversibly interact with cellular macromolecules

AA Brimfield, JC Street - … and Environmental Health, Part A Current …, 1981 - Taylor & Francis
Incubation of 14 C‐labeled cis and trans isomers of chlordane with cofactor‐fortified mouse
hepatic microsomes resulted in binding of insecticide‐derived material to endogenous …