A nonradioactive glycosyltransferase assay is described here. This method takes
advantage of specific phosphatases that can be added into glycosyltransferase reactions to …

purpose. A growing understanding of the molecular events in age-related macular degeneration
(AMD) has lead to targeted therapies for a select group of patients with advanced AMD. …

purpose. Oxidative damage to proteins, lipids, and DNA has been suggested to be a mechanism
for age-related macular degeneration (AMD). The retina is particularly susceptible to …

The proteasome mediates pathways associated with oxidative stress and inflammation, two
pathogenic events correlated with age-related macular degeneration (AMD). In human …

… For these measurements, resolution of αA and insert crystallins from αB crystallin required
the use of large format gels (16 cm × 18 cm) for SDS−PAGE (Pharmacia Hoeffer). Ten …

… For the cell proliferation assays, the cells were seeded into 96-well tissue culture plates
at a density of 31,250 cells/cm 2 in the absence and presence of 10 ng/ml FGF2. Native and …

… cm −1 . The prepared PAPS had a purity greater than 98%. For PAP 35 S preparation, the
reaction was scaled down to 300 μL, and K 2 SO 4 was substituted with 100 μL Na 2 35 SO 4 (…

purpose. Biochemical analysis of age-related macular degeneration (AMD) at distinct stages
of the disease will help further understanding of the molecular events associated with …

… The gel was then subjected to electrophoresis at a constant voltage of 200 volts (~10 v/cm)
with a running buffer (50 mM Tris at pH 8.0) for 25 minutes or till the dye (bromophenol blue) …

Heparan sulfate (HS) is a linear polysaccharide found in the extracellular matrix (ECM) and
on the cell membrane. It plays numerous roles in cellular events, including cell growth, …