Five different members of the flavin-dependent monooxygenase gene family from different
animal species have been described to date. We report the purification and characterization …

The aim of this research was to investigate the influence of benzydamine (BZ) on the in vitro
and in vivo hepatic metabolism of the anthelmintic albendazole (ABZ) in sheep. The …

… (C) Relation between the amount of liver microsomal protein and the relative intensity of
the … 4C) confirmed the increase of the FMO3 protein and the corresponding mRNA. Any …

… Tissue samples were brought to the laboratory for subsequent procedures, which started
within 1 week from sample collection and were carried out between 0 and 4 C. The preparation …

… the maximum FMO enzyme activity was detected at 37 C and at pH 8.0. The apparent Km
value … enzyme activity to zero was 5min at 65 C, 1.5h at 50 C, 48h at 37 C and 20 days at 24 C. …

… All samples were transported to the laboratory in phosphate buffer (0.01 M, pH 7.3) at 4C.
All subsequent operations were performed between 0 and 4C. For each experimental animal, …

… MG96+ (LongGene Scientific) under the following conditions: 10 min at 25 C, 120 min at
37 C and, finally, 5 s at 85 C for enzyme inactivation. The cDNA was stored at −72 C until use. …

… deoxynucleotide triphosphate) at 37C for 1 h and then at 65C for 10 min. … C for 3 min, then
35 cycles of 94C for 30 s, 50C for 30 s, and 72C for 90 s, followed by a final extension at 72C …

… C/EBPα and C/EBPβ bind to C/EBP recognition elements located in CPS1 promoter
region and initiate Cps1 transcription under different physiological conditions [10,76,77]. …

The flavin monooxygenase (FMO) enzyme was discovered in mammalian liver cells that convert
a carcinogenic compound, NN′-dimethylaniline, into a non-carcinogenic compound, N-…