Redox sensing by a Rex‐family repressor is involved in the regulation of anaerobic gene expression in Staphylococcus aureus

…, MC Bauer, C Von Wachenfeldt… - Molecular …, 2010 - Wiley Online Library
An alignment of upstream regions of anaerobically induced genes in Staphylococcus
aureus revealed the presence of an inverted repeat, corresponding to Rex binding sites in …

Structure and functional properties of the Bacillus subtilis transcriptional repressor Rex

…, S Linse, DT Logan, C Von Wachenfeldt - Molecular …, 2008 - Wiley Online Library
The transcription factor Rex has been implicated in regulation of the expression of genes
important for fermentative growth and for growth under conditions of low oxygen tension in …

Genome-wide survey of mRNA half-lives in Bacillus subtilis identifies extremely stable mRNAs

G Hambraeus, C von Wachenfeldt… - Molecular Genetics and …, 2003 - Springer
We have used DNA microarrays to survey rates of mRNA decay on a genomic scale in early
stationary-phase cultures of Bacillus subtilis. The decay rates for mRNAs corresponding to …

Expression, purification, and characterization of Bacillus subtilis cytochromes P450 CYP102A2 and CYP102A3: flavocytochrome homologues of P450 BM3 from …

…, AJ Fulco, MR Cheesman, C Von Wachenfeldt… - Biochemistry, 2004 - ACS Publications
… beginning at 100 C for 0.5 min and increasing to 200 C at a rate of 5 C/min for … C at a rate
of 10 C/min. Injector temperature was set to 250 C and the GC/MS transfer line was set at 280 C

Microsomal P450 2C3 Is Expressed as a Soluble Dimer inEscherichia coliFollowing Modifications of Its N-terminus

C Von Wachenfeldt, TH Richardson, J Cosme… - Archives of biochemistry …, 1997 - Elsevier
… sources of these enzymes for purifithe incorporation of four histidine residues at the C- …
dure (19), and 0.7 units (1 unit will reduce 1 mmol of cytochrome c P450 content and for NADH …

Heterologous expression of human drug-metabolizing enzymes

…, EF Johnson, TH Richardson, C von Wachenfeldt… - Drug Metabolism and …, 1997 - ASPET
… These latter observations led von Wachenfeldt et al. (14) to modify the N terminus of P450
2C3 with the aim of expressing a soluble form of the enzyme. The design of the construct …

Cytochrome bd Biosynthesis inBacillus subtilis: Characterization of thecydABCD Operon

…, KI Yoshida, Y Fujita, C von Wachenfeldt - Journal of …, 1998 - Am Soc Microbiol
Under aerobic conditions Bacillus subtilis utilizes a branched electron transport chain
comprising various cytochromes and terminal oxidases. At present there is evidence for three …

Terminal Oxidases of Bacillus subtilisStrain 168: One Quinol Oxidase, Cytochromeaa3 or Cytochrome bd, Is Required for Aerobic Growth

L Winstedt, C von Wachenfeldt - Journal of bacteriology, 2000 - Am Soc Microbiol
… Winstedt and C. von Wachenfeldt, unpublished data). We do not know under which conditions
… Winstedt L., Yoshida K., Fujita Y., and von Wachenfeldt C. Cytochrome bd biosynthesis in …

Enterococcus faecalis V583 Contains a Cytochrome bd-Type Respiratory Oxidase

…, L Hederstedt, C von Wachenfeldt - Journal of …, 2000 - Am Soc Microbiol
… Winstedt and C. von Wachenfeldt, unpublished data). The cytochrome aa 3 is encoded by
… Winstedt L., Yoshida K., Fujita Y., and von Wachenfeldt C. Cytochrome bd biosynthesis in …

Electrical wiring of live, metabolically enhanced Bacillus subtilis cells with flexible osmium-redox polymers

…, C von Wachenfeldt, C Hägerhäll… - Journal of the …, 2009 - ACS Publications
The present study explores genetic engineering of the respiratory chain and the application
of two different flexible osmium redox polymers to achieve efficient electric communication …