We have designed a modular protein tagging system that allows different functionalities to
be linked onto a single genetic fusion, either in solution, in living cells, or in chemically fixed …

Bioluminescence methodologies have been extraordinarily useful due to their high sensitivity,
broad dynamic range, and operational simplicity. These capabilities have been realized …

Several novel fluorinated fluoresceins (Oregon Green dyes) were prepared by the reaction
of fluororesorcinols with phthalic anhydride and its derivatives. A novel regiospecific …

Background Ratio imaging has received intensive attention in the past few decades. The
growing potential of ratio imaging is significantly limited, however, by the lack of appropriate …

Fluorogenic substrates based on 4-methylumbelliferone (4-MU) have been widely used for
the detection of phosphatase and glycosidase activities. One disadvantage of these …

Using caspase-3 as a model, the authors have developed a strategy for highly sensitive,
homogeneous protease assays suitable for high-throughput, automated applications. The …

Our fundamental understanding of proteins and their biological significance has been
enhanced by genetic fusion tags, as they provide a convenient method for introducing unique …

2,5‐Diphenyloxazoles that embody a dimethylamino group at position 4 of the 5‐phenyl ring
and a sulfonyl group at position 4 of the 2‐phenyl ring were prepared as new fluorescent …

Novel bioluminogenic substrates were designed for probing monoamine oxidase (MAO)
activity based on a simple and effective β-elimination strategy. By modifying the amino group …

A series of 6-benzoxazinylpyridazin-3-ones was prepared and evaluated forinhibition of
cardiac phosphodiesterase (PDE) fraction III in vitro and for positive inotropic activity in vivo. 6-[3…