1. Introduction.–Cannabinoids, which are found only in Cannabis sativa, are secondary
metabolites featuring alkylresorcinol and monoterpene moieties in their molecules. More than 60 …

Cannabinoids, consiting of alkylresorcinol and monoterpene groups, are the unique
secondary metabolites that are found only in Cannabis sativa. Tetrahydrocannabinol (THC), …

Δ 1 -Tetrahydrocannabinolic acid (THCA) synthase is the enzyme that catalyzes oxidative
cyclization of cannabigerolic acid into THCA, the precursor of Δ 1 -tetrahydrocannabinol. We …

… , the recombinant enzyme dissolved in 100 mM sodium citrate buffer (pH 4.0) was irradiated
at 450 nm, and the fluorescence emission spectrum was measured on a Hitachi Model F-…

Tetrahydrocannabinolic acid (THCA) synthase is the enzyme responsible for the production
of tetrahydrocannabinol (THC), the psychoactive component of marijuana (Cannabis sativa L…

We identified a unique enzyme that catalyzes the oxidocyclization of cannabigerolic acid to
cannabidiolic acid (CBDA) in Cannabis sativa L. (CBDA strain). The enzyme, named CBDA …

Numerous plausible hypotheses have been advanced regarding the biogenesis of A'-tetrahydrocannabinol
(A'-THC, la), the psychoactive constituent of marihuana (Cannabis saliva L.); …

… The first round of PCR was performed with gene-specific primer f and adapter primer j in
the presence of poly(dA)-tailed cDNA. The cDNA fragment was obtained by the nested PCR …

Cannabichromenic acid synthase was purified to apparent homogeneity by sequential
column chromatography including DEAE-cellulose, phenyl-Sepharose CL-4B, and …

… (b) Stereo view of the tertiary structure and the F o − F c electron density map (cyan mesh;
contoured at 2 σ level) for the FAD cofactor (cyan) and the covalently bound His114 and …