Mycothiol [2-(N-acetylcysteinyl)amido-2-deoxy-alpha-D-glucopyranosyl- (1-->1)-myo-inositol]
(MSH) has recently been identified as a major thiol in a number of actinomycetes (S. …

A method is described for determination of biological thiols at the picomole level based
upon conversion of thiols to fluorescent derivatives by reaction with monobromobimane and …

Publisher Summary This chapter describes methods for the preparation and high-performance
liquid chromatography (HPLC) analysis of monobromobimane derivatives of low …

Mycothiol (MSH; AcCys-GlcN-Ins) is the major thiol found in Actinobacteria and has many of
the functions of glutathione, which is the dominant thiol in other bacteria and eukaryotes but …

Glutathione is a nearly ubiquitous, low-molecular-mass thiol and antioxidant, but it is conspicuously
absent from most Gram-positive bacteria. We identify here the structure of bacillithiol, …

The bimane fluorescent labels, monobromobimane, dibromobimane, and
monobromotrimethylammoniobimane, are derivatives of syn-9,10-dioxabimane:1,5-diazabicyclo[3.3.0]octa-3,6-…

Bacillithiol (BSH), the α-anomeric glycoside of L-cysteinyl-D-glucosamine with L-malic acid,
is a major low-molecular-weight thiol in Bacillus subtilis and related bacteria. Here, we …

Mycothiol (MSH; 1d-myo-inosityl 2-[N-acetyl-l-cysteinyl]amido-2-deoxy-α-d-glucopyranoside)
is the major low-molecular-weight thiol produced by mycobacteria. Mutants of …

We observed the assembly of a nucleus-like structure in bacteria during viral infection. Using
fluorescence microscopy and cryo-electron tomography, we showed that Pseudomonas …

Entamoeba histolytica was found to grow normally without producing glutathione and the
main enzymes of glutathione metabolism, indicating that glutathione is not essential for many …