Advanced CUBIC protocols for whole-brain and whole-body clearing and imaging

EA Susaki, K Tainaka, D Perrin, H Yukinaga, A Kuno… - Nature protocols, 2015 - nature.com
… rotation at room temperature three times for at least 2 h each time. We typically wash the
sample once for 2 h, once overnight and again once for 2 h. When a sample is stained with PI, …

Development of an optimized backbone of FRET biosensors for kinases and GTPases

…, K Aoki, M Yamada, H Yukinaga… - Molecular biology of …, 2011 - Am Soc Cell Biol
… After 36 h, the cells were metabolically labeled with 32 P i orthophosphate for 2 h and
then lysed. The cell lysates were clarified by centrifugation, and Raichu biosensors were …

[PDF][PDF] Involvement of Ca2+-dependent hyperpolarization in sleep duration in mammals

…, GA Sunagawa, S Shi, EA Susaki, H Yukinaga… - Neuron, 2016 - cell.com
The detailed molecular mechanisms underlying the regulation of sleep duration in mammals
are still elusive. To address this challenge, we constructed a simple computational model, …

In vivo fluorescence resonance energy transfer imaging reveals differential activation of Rho-family GTPases in glioblastoma cell invasion

E Hirata, H Yukinaga, Y Kamioka… - Journal of cell …, 2012 - journals.biologists.com
… 1G,H). These observations demonstrated two modes of glioblastoma invasion: (1)
glioblastoma cells advance along blood vessels straightforwardly and rapidly (Fig. 1I); (2) some …

The closely related RNA helicases, UAP56 and URH49, preferentially form distinct mRNA export machineries and coordinately regulate mitotic progression

T Yamazaki, N Fujiwara, H Yukinaga… - Molecular biology of …, 2010 - Am Soc Cell Biol
… or mitotic cell death (MCD; Duration time) was measured and classified into three groups
as follows: −1.5 h, duration was within 1.5 h; 1.5–3 h, from 1.5 h to 3 h; 3 h+, more than 3 …

Covalent binding and tissue distribution/retention assessment of drugs associated with idiosyncratic drug toxicity

H Takakusa, H Masumoto, H Yukinaga, C Makino… - Drug metabolism and …, 2008 - ASPET
h after administration and was eliminated at 168 h. Imipramine exhibited the retention of
radioactivity in almost all the tissues observed up to 168 h … all tissues at 72 h after administration. …

Wide application of a novel topoisomerase I inhibitor-based drug conjugation technology

…, K Goto, M Goto, M Oitate, H Yukinaga… - Bioorganic & medicinal …, 2016 - Elsevier
To establish a novel and widely applicable payload-linker technology for antibody–drug
conjugates (ADCs), we have focused our research on applying exatecan mesylate (DX-8951f), …

[PDF][PDF] Recording and manipulation of the maternal oxytocin neural activities in mice

H Yukinaga, M Hagihara, K Tsujimoto, HL Chiang… - Current Biology, 2022 - cell.com
… PVHOT often exhibited a cluster of peaks throughout the 12-h recording session, in which …
270 s, and small fractions ranged from 20 min to 1 h (Figure 2E). To analyze the relationships …

On-line capillary isoelectric focusing–mass spectrometry for quantitative analysis of peptides and proteins

Y Kuroda, H Yukinaga, M Kitano, T Noguchi… - … of pharmaceutical and …, 2005 - Elsevier
… 1B), the resolution was calculated as follows: Rs = 1.18 ( T 2 − T 1 ) / ( w h 1 + w h 2 )
where T 1 , T 2 , w h 1 and w h 2 represent the migration times and the peak widths at the half …

Identification of cytochrome P450 3A4 modification site with reactive metabolite using linear ion trap-Fourier transform mass spectrometry

H Yukinaga, T Takami, S Shioyama… - Chemical research in …, 2007 - ACS Publications
… In the (–) substrate control system, H 2 O was added to the reaction mixture instead of the
raloxifene solution. An aliquot of the reaction mixture was analyzed using HPLC/LTQ-FTMS for …