User profiles for James J. Cali
James J. CaliR&D Director, Promega Corporation Verified email at promega.com Cited by 3988 |
[HTML][HTML] Mutations in the bile acid biosynthetic enzyme sterol 27-hydroxylase underlie cerebrotendinous xanthomatosis.
The sterol storage disorder cerebrotendinous xanthomatosis (CTX) is characterized by
abnormal deposition of cholesterol and cholestanol in multiple tissues. Deposition in the central …
abnormal deposition of cholesterol and cholestanol in multiple tissues. Deposition in the central …
[HTML][HTML] Characterization of human sterol 27-hydroxylase. A mitochondrial cytochrome P-450 that catalyzes multiple oxidation reaction in bile acid biosynthesis.
JJ Cali, DW Russell - Journal of Biological Chemistry, 1991 - Elsevier
The enzyme sterol 27-hydroxylase catalyzes the first step in the oxidation of the side chain
of sterol intermediates in the bile acid synthesis pathway. Human sterol 27-hydroxylase …
of sterol intermediates in the bile acid synthesis pathway. Human sterol 27-hydroxylase …
[HTML][HTML] Type VIII adenylyl cyclase. A Ca2+/calmodulin-stimulated enzyme expressed in discrete regions of rat brain.
A cDNA that encodes type VIII adenylyl cyclase has been isolated from two rat brain libraries.
The open reading frame encodes a 1248-amino acid protein predicted to have two sets of …
The open reading frame encodes a 1248-amino acid protein predicted to have two sets of …
Luminogenic cytochrome P450 assays
Luminogenic cytochrome P450 (CYP) assays couple CYP enzyme activity to firefly luciferase
luminescence in a technology called P450-Glo TM (Promega). Luminogenic substrates are …
luminescence in a technology called P450-Glo TM (Promega). Luminogenic substrates are …
Bioluminescent, nonlytic, real-time cell viability assay and use in inhibitor screening
…, P Meisenheimer, G Vidugiris, JJ Cali… - Assay and drug …, 2015 - liebertpub.com
Real-time continuous monitoring of cellular processes offers distinct advantages over traditional
endpoint assays. A comprehensive representation of the changes occurring in live cells …
endpoint assays. A comprehensive representation of the changes occurring in live cells …
[HTML][HTML] A real-time, bioluminescent annexin V assay for the assessment of apoptosis
Apoptosis is an important and necessary cell death program which promotes homeostasis
and organismal survival. When dysregulated, however, it can lead to a myriad of pathologies …
and organismal survival. When dysregulated, however, it can lead to a myriad of pathologies …
[HTML][HTML] A direct capture method for purification and detection of viral nucleic acid enables epidemiological surveillance of SARS-CoV-2
Studies have demonstrated that SARS-CoV-2 RNA can be detected in the feces of infected
individuals. This finding spurred investigation into using wastewater-based epidemiology (…
individuals. This finding spurred investigation into using wastewater-based epidemiology (…
Cloning of the human cholesterol 7α-hydroxylase gene (CYP7) and localization to chromosome 8q11–q12
JC Cohen, JJ Cali, DF Jelinek, M Mehrabian… - Genomics, 1992 - Elsevier
Cholesterol 7α-hydroxylase (7α-hydroxylase) is a microsomal cytochrome P450 that catalyzes
the first step in bile acid synthesis. In this paper, we describe the cloning, characterization, …
the first step in bile acid synthesis. In this paper, we describe the cloning, characterization, …
[HTML][HTML] Selective tissue distribution of G protein gamma subunits, including a new form of the gamma subunits identified by cDNA cloning.
JJ Cali, EA Balcueva, I Rybalkin… - Journal of Biological …, 1992 - Elsevier
The GTP-binding regulatory proteins (G proteins) that transduce signals from receptors to
effectors are composed of alpha, beta, and gamma subunits. Whereas the role of alpha …
effectors are composed of alpha, beta, and gamma subunits. Whereas the role of alpha …
N-Alkylated 6′-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays
…, JW Shultz, MG Wood, J Osterman, JJ Cali… - Biochemistry, 2008 - ACS Publications
A set of 6′-alkylated aminoluciferins are shown to be bioluminescent substrates for Ultra-Glo
and QuantiLum luciferases. These studies demonstrate that both the engineered and wild-…
and QuantiLum luciferases. These studies demonstrate that both the engineered and wild-…