User profiles for Mary Sobol

Mary Sobol

Promega
Verified email at promega.com
Cited by 400

Luminogenic cytochrome P450 assays

JJ Cali, D Ma, M Sobol, DJ Simpson… - Expert opinion on …, 2006 - Taylor & Francis
Luminogenic cytochrome P450 (CYP) assays couple CYP enzyme activity to firefly luciferase
luminescence in a technology called P450-Glo TM (Promega). Luminogenic substrates are …

Proluciferin acetals as bioluminogenic substrates for cytochrome P450 activity and probes for CYP3A inhibition

PL Meisenheimer, HT Uyeda, D Ma, M Sobol… - Drug Metabolism and …, 2011 - ASPET
Cytochrome P450 (P450) assays use probe substrates to interrogate the influence of new
chemical entities toward P450 enzymes. We report the synthesis and study of a family of …

Bioluminescent cell-based NAD (P)/NAD (P) H assays for rapid dinucleotide measurement and inhibitor screening

J Vidugiriene, D Leippe, M Sobol… - Assay and drug …, 2014 - liebertpub.com
The central role of nicotinamide adenine dinucleotides in cellular energy metabolism and
signaling makes them important nodes that link the metabolic state of cells with energy …

Self‐immolative bioluminogenic quinone luciferins for NAD (P) H assays and reducing capacity‐based cell viability assays

W Zhou, D Leippe, S Duellman, M Sobol… - …, 2014 - Wiley Online Library
Highly sensitive self‐cleavable trimethyl lock quinone‐luciferin substrates for diaphorase were
designed and synthesized to measure NAD(P)H in biological samples and monitor viable …

Bioluminescent assays for glucose and glutamine metabolism: High-throughput screening for changes in extracellular and intracellular metabolites

D Leippe, M Sobol, G Vidugiris… - … Life Sciences R&D, 2017 - journals.sagepub.com
Cancer cell metabolism is a complex, dynamic network of regulated pathways. Interrogation
of this network would benefit from rapid, sensitive techniques that are adaptable to high-…

[PDF][PDF] CYP3A4 P450-GloŽ Assays with Luciferin-IPA: The Most Sensitive and Selective Bioluminescent CYP3A4 Assay

JJ Cali, M Sobol, D Ma, HT Uyeda… - … . Available online at …, 2009 - promega.com
The cytochromes P450 (CYPs) are the main enzyme family for metabolism of therapeutic
drugs (1). CYP3A4 is the most active drug metabolizing CYP, and it plays a prominent role in …

[PDF][PDF] Comparison of inhibition of CYP1A2, 2C9 and 3A4 using human liver microsomes and hepatocytes

T Moeller, T Worzella, M Sobol, D Ma, JJ Cali… - … , Toronto. Mostafavi SA …, 2013 - veritastk.co.jp
Determination of inhibition of drug metabolizing enzymes is a key component to assessing
the risk of drug-drug interactions (DDI). Inhibition may lead to greater bioavailability and lower …

Automated luminescence-based cytochrome P450 profiling using a simple, elegant robotic platform

B Larson, P Banks, JJ Cali, M Sobol… - JALA: Journal of the …, 2011 - journals.sagepub.com
The determination of inhibitory effects that lead compounds have on cytochrome P450 (CYP)
ezymes is an important part of today's drug discovery process. Assays can be performed …

[PDF][PDF] Bioluminescent Cytochrome P450 Assays.

A Bosetti, M Sobol, D Ma, T Good, D Liu, JJ Cali - Pharmacology, 2005 - academia.edu
P450-Glo™ Luminescent P450 assays utilize derivatives of beetle luciferin as luminogenic
probe P450 substrates. Luciferin is a substrate for the light generating reaction of firefly …

[PDF][PDF] Automation of a Multiplexed Cell-based Assay to Measure Simultaneously Inhibition and Induction of the Cytochrome P450 Isoform 3A4 by Small Molecule …

…, P Banks, J Cali, M Sobol - poster presentation at 16th …, 2009 - m.art-studio-tour.com
A total of 54 compounds were tested in the study. Seven compounds were run per plate,
plus a control compound (Rifampicin), creating eight 96-well plates tested. The final plate …