User profiles for Mary Sobol
Mary SobolPromega Verified email at promega.com Cited by 400 |
Luminogenic cytochrome P450 assays
Luminogenic cytochrome P450 (CYP) assays couple CYP enzyme activity to firefly luciferase
luminescence in a technology called P450-Glo TM (Promega). Luminogenic substrates are …
luminescence in a technology called P450-Glo TM (Promega). Luminogenic substrates are …
Proluciferin acetals as bioluminogenic substrates for cytochrome P450 activity and probes for CYP3A inhibition
Cytochrome P450 (P450) assays use probe substrates to interrogate the influence of new
chemical entities toward P450 enzymes. We report the synthesis and study of a family of …
chemical entities toward P450 enzymes. We report the synthesis and study of a family of …
Bioluminescent cell-based NAD (P)/NAD (P) H assays for rapid dinucleotide measurement and inhibitor screening
J Vidugiriene, D Leippe, M Sobol… - Assay and drug …, 2014 - liebertpub.com
The central role of nicotinamide adenine dinucleotides in cellular energy metabolism and
signaling makes them important nodes that link the metabolic state of cells with energy …
signaling makes them important nodes that link the metabolic state of cells with energy …
Self‐immolative bioluminogenic quinone luciferins for NAD (P) H assays and reducing capacity‐based cell viability assays
Highly sensitive self‐cleavable trimethyl lock quinone‐luciferin substrates for diaphorase were
designed and synthesized to measure NAD(P)H in biological samples and monitor viable …
designed and synthesized to measure NAD(P)H in biological samples and monitor viable …
Bioluminescent assays for glucose and glutamine metabolism: High-throughput screening for changes in extracellular and intracellular metabolites
D Leippe, M Sobol, G Vidugiris… - … Life Sciences R&D, 2017 - journals.sagepub.com
Cancer cell metabolism is a complex, dynamic network of regulated pathways. Interrogation
of this network would benefit from rapid, sensitive techniques that are adaptable to high-…
of this network would benefit from rapid, sensitive techniques that are adaptable to high-…
[PDF][PDF] CYP3A4 P450-GloŽ Assays with Luciferin-IPA: The Most Sensitive and Selective Bioluminescent CYP3A4 Assay
The cytochromes P450 (CYPs) are the main enzyme family for metabolism of therapeutic
drugs (1). CYP3A4 is the most active drug metabolizing CYP, and it plays a prominent role in …
drugs (1). CYP3A4 is the most active drug metabolizing CYP, and it plays a prominent role in …
[PDF][PDF] Comparison of inhibition of CYP1A2, 2C9 and 3A4 using human liver microsomes and hepatocytes
T Moeller, T Worzella, M Sobol, D Ma, JJ Cali… - … , Toronto. Mostafavi SA …, 2013 - veritastk.co.jp
Determination of inhibition of drug metabolizing enzymes is a key component to assessing
the risk of drug-drug interactions (DDI). Inhibition may lead to greater bioavailability and lower …
the risk of drug-drug interactions (DDI). Inhibition may lead to greater bioavailability and lower …
Automated luminescence-based cytochrome P450 profiling using a simple, elegant robotic platform
The determination of inhibitory effects that lead compounds have on cytochrome P450 (CYP)
ezymes is an important part of today's drug discovery process. Assays can be performed …
ezymes is an important part of today's drug discovery process. Assays can be performed …
[PDF][PDF] Bioluminescent Cytochrome P450 Assays.
P450-Glo™ Luminescent P450 assays utilize derivatives of beetle luciferin as luminogenic
probe P450 substrates. Luciferin is a substrate for the light generating reaction of firefly …
probe P450 substrates. Luciferin is a substrate for the light generating reaction of firefly …
[PDF][PDF] Automation of a Multiplexed Cell-based Assay to Measure Simultaneously Inhibition and Induction of the Cytochrome P450 Isoform 3A4 by Small Molecule …
A total of 54 compounds were tested in the study. Seven compounds were run per plate,
plus a control compound (Rifampicin), creating eight 96-well plates tested. The final plate …
plus a control compound (Rifampicin), creating eight 96-well plates tested. The final plate …