… cm-') after conversion into its cyanide complex by addition of 0.4mM-KCN (N. Oshino,
unpublished work). Free catalase in the peroxisomal suspension, which was released from broken …

The extinction of fluorescence of scopoletin during its oxidation by horseradish peroxidase (HPO)
provides a highly sensitive and specific assay for small quantities of peroxide in …

… NADH- and N ADPH-cytochrome c reductase activities were measured according to Dallner
( 2-) and Phillips and Langdon (19 ), respec tively, except that 0.1 M Tris-HCl buffer, pH 7.2, …

… The method is based on a relationship between the ratio of H 2 O 2 generaztion rate ( dx
n dt ) to catalase-heme concentration (e) and the concentration of methanol ( a 1 2 ) which …

The importance of measuring intracellular oxygen concentrations in tissues has, over the
years, emerged as a basic parameter in the physiology and biochemistry of living tissues. The …

… Chance & N. Oshino, unpublished work) can respond to ethanol and methanol indicates
that catalase actually functions in ethanol and methanol metabolism, though the absolute rates …

1. The primary intermediate of catalase and hydrogen peroxide was identified and investigated
in peroxisome-rich mitochondrial fractions of rat liver. On the basis of kinetic constants …

… Anaerobic heart was obtained with the perfusate equilibrated with 95% N,+ 5% COt. The
perfusate was not recycled in the flow-through system: its temperature was maintained at 25-26…

… of O 2 (P 50 ) which is required for half maximal deoxygenation (or oxygenation) of myoglobin
in perfused heart is found to be 2.4 mm Hg at room temperature and the Hill constant, n, is …

… The, method of Dallnw (17), in which both subfractions could be prcparcd in a much shortw
time, n-as thcrefore employcbd, resulting in 110 significant loss of the activity. Th(, RNA cont,…