The enzymic isolation of adult rat hepatocytes in a functional and viable state

JR Fry, CA Jones, P Wiebkin, P Bellemann… - Analytical …, 1976 - Elsevier
Various methods for the preparation of rat hepatocyte suspensions have been compared
with regard to cell yield and viability index. A modified method of collagenase/hyaluronidase …

Biphenyl metabolism in isolated rat hepatocytes: effect of induction and nature of the conjugates

P Wiebkin, JR Fry, CA Jones, RK Lowing… - Biochemical …, 1978 - Elsevier
Biphenyl is metabolised by untreated isolated rat hepatocytes via hydroxylation at the 4-position
followed by conjugation with sulphate and glucuronic acid. At a substrate concentration …

The microsomal metabolism of the organometallic derivatives of the Group-IV elements, germanium, tin and lead

RA Prough, MA Stalmach, P Wiebkin… - Biochemical …, 1981 - portlandpress.com
The NADPH- and oxygen-dependent microsomal metabolism of the di-, tri- and tetra-ethyl-substituted
derivatives of germanium, tin and lead was shown to give rise to ethylene as a …

The metabolism of biphenyl by isolated viable rat hepatocytes

P Wiebkin, JR Fry, CA Jones, R Lowing, JW Bridges - Xenobiotica, 1976 - Taylor & Francis
… The reaction was initiated by the addition of 0-27pCi of [14C]biphenyl in lop1 of
dimethylformamide (equivalent to a final biphenyl concentration of 1 3 0 p ~ in the cell incubation …

A comparison of drug-metabolizing capability in isolated viable rat hepatocytes and renal tubule fragments

JR Fry, P Wiebkin, J Kao, CA Jones, J Gwynn… - Xenobiotica, 1978 - Taylor & Francis
… However, the fact that kidney cells do possess a cytochrome P-450 system capable of
metabolizing xenobiotics is of some importance, because of the possibility that even a low level of …

Oxidative Metabolism of N-Isopropyl-α-(2-methylazo)-p-toluamide (Azoprocarbazine) by Rodent Liver Microsomes

P Wiebkin, RA Prough - Cancer Research, 1980 - AACR
… cytochrome P-450 inhibitors. Azoprocarbazine elicits a very weak spectral complex (type II)
with rat liver microsomal cytochrome P-450. These results strongly suggest that cytochrome P

7-Ethoxycoumarin O-deethylase induction by phenobarbitone and 1, 2-benzanthracene in primary maintenance cultures of adult rat hepatocytes

JR Fry, P Wiebkin, JW Bridges - Biochemical Pharmacology, 1980 - Elsevier
The microsomal monooxygenase system of adult rat hepatocytes in short-term non-proliferating
culture could be induced by phenobarbitone and benzanthracene. Differences in the …

The metabolism and toxicity of some organotin compounds in isolated rat hepatocytes

P Wiebkin, RA Prough, JW Bridges - Toxicology and applied pharmacology, 1982 - Elsevier
… While diethyltin dichloride could also cause the formation of cytochrome P-420 from
cytochromes P-450, it was much less effective. Similar studies carried out in isolated hepatocyte …

Inhibition of glutathione-related enzymes and cytotoxicity of ethacrynic acid and cyclosporine

DW Hoffman, P Wiebkin, LP Rybak - Biochemical pharmacology, 1995 - Elsevier
Glutathione (GSH) is an endogenous thiol that detoxifies active oxygen and reactive species
formed during intermediary metabolism and drug detoxification. Compounds with a range of …

Effect of various metabolic inhibitors on biphenyl metabolism in isolated rat hepatocytes

P Wiebkin, GL Parker, JR Fry, JW Bridges - Biochemical Pharmacology, 1979 - Elsevier
… The uncoupling of the microsomai respiratory system rather than a mitochondrial effect is
presumably responsible for the decrease in the P-450 mediated hydroxylation P-450 mediated …