Utility of non-human primates in drug development: Comparison of non-human primate and human drug-metabolizing cytochrome P450 enzymes
Y Uno, S Uehara, H Yamazaki - Biochemical pharmacology, 2016 - Elsevier
Cynomolgus monkeys (Macaca fascicularis, an Old World Monkey) have been widely used
as a non-human primate model in preclinical studies because of their genetic and …
as a non-human primate model in preclinical studies because of their genetic and …
[HTML][HTML] An improved TK-NOG mouse as a novel platform for humanized liver that overcomes limitations in both male and female animals
S Uehara, Y Higuchi, N Yoneda, K Kawai… - Drug Metabolism and …, 2022 - Elsevier
We developed a novel immunodeficient NOG mouse expressing HSVtk mutant clone 30
cDNA under the control of mouse transthyretin gene enhancer/promoter (NOG-TKm30) to …
cDNA under the control of mouse transthyretin gene enhancer/promoter (NOG-TKm30) to …
In Vivo and In Vitro Induction of Cytochrome P450 3A4 by Thalidomide in Humanized-Liver Mice and Experimental Human Hepatocyte HepaSH cells
S Uehara, N Murayama, Y Higuchi… - Chemical Research …, 2024 - ACS Publications
Autoinduction of cytochrome P450 (P450) 3A4-mediated metabolism of thalidomide was
investigated in humanized-liver mice and human hepatocyte-derived HepaSH cells. The mean …
investigated in humanized-liver mice and human hepatocyte-derived HepaSH cells. The mean …
Immunochemical detection of cytochrome P450 enzymes in liver microsomes of 27 cynomolgus monkeys
S Uehara, N Murayama, Y Nakanishi, DC Zeldin… - … of Pharmacology and …, 2011 - ASPET
The cynomolgus monkey is widely used as a primate model in preclinical studies because
of its evolutionary closeness to humans. Despite their importance in drug metabolism, the …
of its evolutionary closeness to humans. Despite their importance in drug metabolism, the …
[HTML][HTML] Humanized liver TK-NOG mice with functional deletion of hepatic murine cytochrome P450s as a model for studying human drug metabolism
S Uehara, Y Iida, M Ida-Tanaka, M Goto, K Kawai… - Scientific Reports, 2022 - nature.com
Chimeric TK-NOG mice with a humanized liver (normal Hu-liver) are a unique animal model
for predicting drug metabolism in humans. However, residual mouse hepatocytes …
for predicting drug metabolism in humans. However, residual mouse hepatocytes …
[HTML][HTML] HepaSH cells: Experimental human hepatocytes with lesser inter-individual variation and more sustainable availability than primary human hepatocytes
S Uehara, Y Higuchi, N Yoneda, R Ito… - Biochemical and …, 2023 - Elsevier
Primary human hepatocytes (PHHs) have been commonly used as the gold standard in many
drug metabolism studies, regardless of having large inter-individual variation. These inter-…
drug metabolism studies, regardless of having large inter-individual variation. These inter-…
Genetic variants of CYP3A4 and CYP3A5 in cynomolgus and rhesus macaques
Cynomolgus and rhesus macaques are frequently used in preclinical trials due to their close
evolutionary relationships to humans. We conducted an initial screening for genetic variants …
evolutionary relationships to humans. We conducted an initial screening for genetic variants …
Newly identified tree shrew cytochrome P450 2B6 (CYP2B6) and pig CYP2B6b are functional drug-metabolising enzymes
Y Uno, G Ushirozako, S Uehara, N Murayama… - Xenobiotica, 2022 - Taylor & Francis
Tree shrews have high phylogenetic affinity to humans and are used in various fields of
biomedical research, especially hepatitis virus infection; however, cytochromes P450 (P450s or …
biomedical research, especially hepatitis virus infection; however, cytochromes P450 (P450s or …
Novel marmoset cytochrome P450 2C19 in livers efficiently metabolizes human P450 2C9 and 2C19 substrates, S-warfarin, tolbutamide, flurbiprofen, and omeprazole
S Uehara, Y Uno, T Inoue, M Kawano, M Shimizu… - Drug metabolism and …, 2015 - ASPET
The common marmoset (Callithrix jacchus), a small New World monkey, has the potential
for use in human drug development due to its evolutionary closeness to humans. Four novel …
for use in human drug development due to its evolutionary closeness to humans. Four novel …
Cytochrome P450-dependent drug oxidation activities and their expression levels in liver microsomes of chimeric TK-NOG mice with humanized livers
S Uehara, N Yoneda, Y Higuchi, H Yamazaki… - Drug Metabolism and …, 2022 - Elsevier
Hepatic cytochrome P450 (P450)-dependent drug oxidation activity has not been completely
characterized in chimeric TK-NOG mice with humanized livers (humanized liver mice). In …
characterized in chimeric TK-NOG mice with humanized livers (humanized liver mice). In …