Potential impact of steatosis on cytochrome P450 enzymes of human hepatocytes isolated from fatty liver grafts

MT Donato, A Lahoz, N Jimenez, G Pérez… - Drug metabolism and …, 2006 - ASPET
Liver grafts discarded for transplantation because of macrosteatosis can constitute a
valuable source of human hepatocytes for in vitro metabolic and pharmacotoxicological …

Effects of steatosis on drug-metabolizing capability of primary human hepatocytes

MT Donato, N Jimenez, A Serralta, J Mir, JV Castell… - Toxicology in vitro, 2007 - Elsevier
The suitability of liver grafts discarded for transplantation because of macrosteatosis for
preparing human hepatocyte cultures for in vitro drug metabolism studies has been …

Effects of fatty acids and ketone bodies on cytochromes P450 2B, 4A, and 2E1 expression in primary cultured rat hepatocytes

RC Zangar, RF Novak - Archives of biochemistry and biophysics, 1997 - Elsevier
CYP2B, CYP4A, and CYP2E1 mRNA levels are elevated in response to pathophysiological
conditions, such as diabetes, high-fat diet, and fasting, in which lipids and ketone bodies are …

CYP4F3B expression is associated with differentiation of HepaRG human hepatocytes and unaffected by fatty acid overload

S Madec, V Cerec, E Plée-Gautier, J Antoun… - Drug metabolism and …, 2011 - ASPET
Fatty acid microsomal ω-oxidation involves cytochrome P450 enzymes. Some of them
belonging to the CYP4F3 family are mainly expressed in the liver, making this organ a major …

Reduction in hepatic cytochrome P-450 is correlated to the degree of liver fat content in animal models of steatosis in the absence of inflammation

I Leclercq, Y Horsmans, JP Desager, N Delzenne… - Journal of …, 1998 - Elsevier
Background/Aim: Fatty liver has been associated with an increased risk of primary graft non-
function and drug toxicity. However, these effects have been observed mainly in fatty liver …

Cytochrome p450 and steatosis

MJ Gomez-Lechon, R Jover… - Current drug …, 2009 - ingentaconnect.com
The term fatty liver identifies a liver in which lipids account for more than 5% of the liver's wet
weight. When fat accumulates, the lipids primarily stored as triglycerides (TG) result in …

Regulation of CYP2E1 by ethanol and palmitic acid and CYP4A11 by clofibrate in primary cultures of human hepatocytes

JL Raucy, J Lasker, K Ozaki, V Zoleta - Toxicological Sciences, 2004 - academic.oup.com
Abstract CYP2E1 and CYP4A11 are cytochrome P450 enzymes that are regulated by
physiological conditions including diabetes and fasting. In addition, the xenochemical …

Differential effects of dietary fatty acids on the regulation of CYP2E1 and protein kinase C in human hepatoma HepG2 cells

M Sung, I Kim, M Park, Y Whang, M Lee - Journal of medicinal food, 2004 - liebertpub.com
We investigated the effects of different fatty acids (FAs) or with different degrees of
unsaturation on cytochrome P450 2E1 (CYP2E1) induction and protein kinase C (PKC) …

Determination of cytochrome P450 1A2 and cytochrome P450 3A4 induction in cryopreserved human hepatocytes

D Roymans, C Van Looveren, A Leone… - Biochemical …, 2004 - Elsevier
Freshly prepared human hepatocytes are considered as the 'gold standard'for in vitro testing
of drug candidates. However, several disadvantages are associated with the use of this …

Cytochromes P450 with bisallylic hydroxylation activity on arachidonic and linoleic acids studied with human recombinant enzymes and with human and rat liver …

J Bylund, T Kunz, K Valmsen, EH Oliw - Journal of Pharmacology and …, 1998 - ASPET
Bisallylic carbons of polyunsaturated fatty acids can be hydroxylated in NADPH-dependent
reactions in liver microsomes. Human recombinant cytochromes P450 and human and rat …