Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Drug Metabolism & Disposition
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Drug Metabolism & Disposition

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit dmd on Facebook
  • Follow dmd on Twitter
  • Follow ASPET on LinkedIn
Abstract

Purification and characterization of human kidney cytosolic cysteine conjugate beta-lyase activity.

L H Lash, R M Nelson, R A Van Dyke and M W Anders
Drug Metabolism and Disposition January 1990, 18 (1) 50-54;
L H Lash
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R M Nelson
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R A Van Dyke
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
M W Anders
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

The central role of cysteine conjugate beta-lyase (beta-lyase) in the bioactivation of nephrotoxic halogenated hydrocarbons and the possibility of human exposure to these chemicals has focused interest on the beta-lyase from human kidney. Human kidney tissue was collected as surgical waste material, and subcellular fractions were isolated by differential centrifugation. Human beta-lyase activity, determined with S-(2-benzothiazolyl)-L-cysteine (BTC) as the substrate, was present in the cytosolic, mitochondrial, and microsomal fractions, but was highest in the cytosolic fraction. Activity in human kidney cytosol was about 10% of that present in rat kidney cytosol. Human kidney cytosolic beta-lyase activity, with BTC as the substrate, was not stimulated by pyridoxal phosphate or by exogenous 2-keto acids. Cytosolic beta-lyase activity was purified 280-fold with a yield of 12% from human kidneys unsuitable for transplantation. The beta-lyase activity copurified with cytosolic glutamine transaminase K and exhibited a molecular mass of 85 kDa on a Sephacryl 5300 column and a subunit molecular mass of 45 kDa by gel electrophoresis. Both BTC and its homocysteine analogue S-(2-benzothiazolyl)-L-homocysteine were excellent substrates, exhibiting Km and kcat values of 0.97 mM and 2.78 mM and 9.35 min-1 and 6.90 min-1, respectively. beta-Lyase activity was inhibited by aminooxyacetic acid, indicating that the human cytosolic enzyme contains pyridoxal phosphate, and by the nephrotoxins S-(1,2-dichlorovinyl)-L-cysteine and S-(1,2-dichlorovinyl)-L-homocysteine, which serve as alternative substrates.(ABSTRACT TRUNCATED AT 250 WORDS)

 

DMD articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Drug Metabolism and Disposition
Vol. 18, Issue 1
1 Jan 1990
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author
  • Back Matter (PDF)
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Drug Metabolism & Disposition article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Purification and characterization of human kidney cytosolic cysteine conjugate beta-lyase activity.
(Your Name) has forwarded a page to you from Drug Metabolism & Disposition
(Your Name) thought you would be interested in this article in Drug Metabolism & Disposition.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Abstract

Purification and characterization of human kidney cytosolic cysteine conjugate beta-lyase activity.

L H Lash, R M Nelson, R A Van Dyke and M W Anders
Drug Metabolism and Disposition January 1, 1990, 18 (1) 50-54;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Abstract

Purification and characterization of human kidney cytosolic cysteine conjugate beta-lyase activity.

L H Lash, R M Nelson, R A Van Dyke and M W Anders
Drug Metabolism and Disposition January 1, 1990, 18 (1) 50-54;
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About DMD
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Journal of Pharmacology and Experimental Therapeutics
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-009X (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics