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Abstract

Metabolism of antiparkinson agent dopazinol by rat liver microsomes.

K P Vyas, P H Kari, H G Ramjit, S M Pitzenberger and M Hichens
Drug Metabolism and Disposition November 1990, 18 (6) 1025-1030;
K P Vyas
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P H Kari
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H G Ramjit
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S M Pitzenberger
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M Hichens
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Abstract

Metabolism of dopazinol (DZ) by liver microsomes from control and phenobarbital- and 3-methylcholanthrene-treated rats has been investigated. Liver microsomes from control and treated rats metabolized DZ to N-despropyl-DZ (39-53% of total metabolites); 8-hydroxy-DZ, a catechol metabolite (32-39%); and 5- or 6-hydroxy-DZ (12-20%). The last metabolite was identified as its dehydration product 5,6-dehydro-DZ. N-Dealkylation was favored only slightly over catechol formation (ratio = 1.2) by liver microsomes from control and phenobarbital-treated rats, whereas with liver microsomes from 3-methylcholanthrene-treated rats, N-dealkylation predominated (ratio = 1.7). Liver microsomes from control rats metabolized DZ at a rate of 0.86 nmol/nmol cytochrome P-450/min. Pretreatment of rats with phenobarbital or 3-methylcholanthrene stimulated rates of metabolism by 2.4- and 3-fold, respectively. Metabolism of DZ was inhibited by SKF 525-A, methimazole, and thiobenzamide. SKF 525-A completely inhibited metabolism of DZ, while methimazole and thiobenzamide, two alternate substrates of the microsomal flavin-containing monooxygenase (MFMO) inhibited N-dealkylation only. These results indicated that while the cytochrome P-450-dependent monooxygenase is the primary enzyme system in DZ oxidation, the MFMO also catalyzes the N-dealkylation reaction. The catechol metabolite was converted to isomeric O-methylated derivatives in approximately 1:1 ratio by purified catechol-O-methyl transferase or 105,000g liver cytosol. The late eluting isomer was 8-methoxy-DZ.

 

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Drug Metabolism and Disposition
Vol. 18, Issue 6
1 Nov 1990
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Abstract

Metabolism of antiparkinson agent dopazinol by rat liver microsomes.

K P Vyas, P H Kari, H G Ramjit, S M Pitzenberger and M Hichens
Drug Metabolism and Disposition November 1, 1990, 18 (6) 1025-1030;

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Abstract

Metabolism of antiparkinson agent dopazinol by rat liver microsomes.

K P Vyas, P H Kari, H G Ramjit, S M Pitzenberger and M Hichens
Drug Metabolism and Disposition November 1, 1990, 18 (6) 1025-1030;
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