Abstract
The metabolism of cinobufagin (CB) in rat liver microsomes was studied. By comparison of retention times and fragmentation patterns obtained by thermospray LC/MS with those of authentic standards, four metabolites of CB were also identified, in addition to deacetylCB (M1) and 3-epideacetylCB (M2), which have been reported previously. The additional four metabolites were identified to be 3-epiCB (M4), 3-ketoCB (M5), 3-keto-16 beta-OH-deacetylCB (M3), and 3-keto-16 alpha-OH-deacetylCB (M6). The primary metabolic products of CB were M1 and M2, with a small amount of M4 and trace amounts of M3, M5, and M6. These results indicate that major metabolic routes of CB were deacetylation at the 16-position and epimerization at the 3-position via the 3-keto intermediate.
DMD articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|
