Abstract
Measurement of intracellular drug levels in cell culture systems can be of predictive value in establishing rational clinical dosage schedules. Such in vitro measurements carried out with anti-HIV agents of the 2′,3′-dideoxynucleoside (ddN) class have shown that many of the pharmacologically active ddNTP metabolites of these agents have relatively long intracellular half-lives and little or no host-cell cytotoxicity. As a consequence, replication of drug-exposed cells continues at an unperturbed rate so that a systematic dilution error occurs in the measurement of ddNTP decay half-times. The aim of this study is to present a simple general formulation for the correction of measured t1/2-values for ddNTPs and for other agents with similar intracellular pharmacokinetic properties. Two factors of practical interest emerge: first, the error is greater for agents with slow intracellular clearance rates than for agents with rapid rates; and second, for cell lines with long doubling times, the measured t1/2-values approach more closely to the true t1/2-values, until with the extreme case (quiescent or "G0" cells), the observed and true decay times are identical. The greatest dilution errors are seen with adenodine-based agents such as ddATP and 2′-F-ddATP, while the smallest errors are seen with rapidly cleared agents of the dideoxythymidine class.
Footnotes
-
Send reprint requests to: Dr. David G. Johns, Building 37, Room 5B22, Laboratory of Medicinal Chemistry, Division of Basic Sciences, National Cancer Institute/National Institutes of Health, Bethesda, MD 20892-4255.
-
Received September 11, 1996; accepted March 25, 1997.
- Abbreviations used are::
- ddN
- 2′,3′-dideoxynucleoside
- HIV
- human immunodeficiency virus
- ddNTP
- ddN-5′-triphosphate
- ddATP
- 2′,3′-dideoxyadenosine-5′-triphosphate
- F-ddATP
- 2′-β-fluoro-ddATP
- ddI
- 2′,3′-dideoxyinosine
- F-ddA
- 2′-β-fluoro-2′,3′-dideoxyadenosine
- ddGTP
- 2′,3′-dideoxyguanosine-5′-triphosphate
- PBM cells
- peripheral blood mononuclear cells
- D4T
- 2′,3′-didehydro-3′-deoxythymidine
- PHA
- phytohemagglutinin
- tm
- measured (uncorrected)t1/2
- tD
- cell doubling time
- 3TC-TP
- 2′-deoxy-3′-thiacytidine-5′-triphosphate
- AZT
- 3′-azido-3′-deoxythymidine
- ddC
- 2′,3′-dideoxycytidine
- The American Society for Pharmacology and Experimental Therapeutics
DMD articles become freely available 12 months after publication, and remain freely available for 5 years.Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page.
|