Identification of Glycine Conjugates, Including Hippurate, as Urinary Metabolites of Dichloroacetate
Abstract
Pathways of metabolism of dichloroacetate (DCA), an investigational drug for the treatment of lactic acidosis in humans and a rodent hepatocarcinogen, are poorly understood. In this study, rats were given, by gavage, one or two 50 mg/kg doses of NaDCA. DCA labeled with14C (carboxy carbon) or13C (both carbons) was used in studies of disposition and pharmacokinetics, respectively. The effect of fasting for 14 hr before dosing was studied. Expired air, urine, feces, and tissues were collected from [14C]DCA-dosed rats. Urine was analyzed by HPLC, GC/MS, and NMR spectroscopy. Plasma samples were analyzed by GC/MS. DCA plasma elimination half-lives were 0.1 ± 0.02 and 5.4 ± 0.8 hr in young adult rats (180–265 g, 3–4 months of age) given one or two doses of DCA, respectively, and 9.7 ± 1 hr in large, 16-month-old rats given two DCA doses. The percentage of the DCA dose excreted as CO2varied from 17 to 46% and was lower (p < 0.001) in fed rats, compared with rats fasted overnight before dosing. Urine contained DCA and DCA metabolites, including oxalate, glyoxylate, and conjugated glycine (mainly hippurate and phenylacetylglycine). More unchanged DCA was excreted by large rats pretreated with DCA (mean, 20.2% of the dose) than by young adult rats given one dose of DCA (mean, 0.5%). This study confirmed that CO2, glycine, and oxalate are major products of DCA metabolism, it demonstrated that one dose of DCA altered the elimination of a subsequent dose, and it showed that age or body size, as well as access to food, significantly affected DCA metabolism in rats.
Footnotes
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Send reprint requests to: Dr. Margaret O. James, Department of Medicinal Chemistry, P.O. Box 100485, College of Pharmacy, University of Florida, Gainesville FL 32610-0485. e-mail:MOJames{at}mc.cop.ufl.edu
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↵1 Present address: American Cyanamid, Princeton, NJ.
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↵2 Present address: Abbott Pharmaceuticals, Chicago, IL.
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This work was supported in part by National Institutes of Health Grant ES07375. Preliminary accounts of portions of this work were presented at meetings of the International Society for the Study of Xenobiotics (Raleigh, NC, 1994; Hilton Head, SC, 1997).
- Abbreviations used are::
- DCA
- dichloroacetate
- AIC
- Akaike’s information criterion
- Received February 13, 1998.
- Accepted July 9, 1998.
- The American Society for Pharmacology and Experimental Therapeutics
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