Abstract
We have already reported that the quinol formation from some para-alkylphenols, which is a novel metabolic pathway catalyzed by cytochrome P-450, occurs in a rat liver microsomal system (Ohe et al., 1997). In the present study, we investigated whether estrone and 17β-estadiol, each of which contains ap-alkylphenol moiety, are also oxidized into the corresponding quinols by cytochrome P-450. Six recombinant human cytochrome P-450 enzymes, CYP1A1, CYP1A2, CYP2B6, CYP2C9, CYP2E1, and CYP3A4, were tested. The results show that estrone and 17β-estadiol were converted into the corresponding quinols by CYP1A1, CYP2B6, and CYP2E1.
Footnotes
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Send reprint requests to: Tadahiko Mashino, Kyoritsu College of Pharmacy, 1-5-30 Shibakoen, Minato-ku, Tokyo 105-8512, Japan. E-mail: mashino-td{at}kyoritsu-ph.ac.jp
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↵2 Present address: Tsukuba Research Institute, Banyu Pharmaceutical Co., Ltd., Okubo 3, Tsukuba 300-2611, Japan.
- Abbreviations used are::
- P-450
- cytochrome P-450
- G-6-P
- glucose-6-phosphate
- GC-SIM
- gas chromatography-single ion monitor
- Received July 7, 1999.
- Accepted October 12, 1999.
- The American Society for Pharmacology and Experimental Therapeutics
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