Abstract
Human genomic libraries were screened to identifyCYP2G-related cytochrome P450 genes. A genomic fragment comprising exons 7 through 9 of CYP2GP1 and exons 6 through 9 of a previously unidentified CYP2A gene, designated CYP2A7P2, was isolated from an EMBL3 library; the two genes were arranged in outward opposite directions with about 8 kbp of intervening sequence. The same structure was also detected in a bacteriophage P1 clone, which contained a full-lengthCYP2GP1 gene, exons 6 through 9 ofCYP2A7P2, and the CYP2B7 gene. However, additional CYP2A-related exons as well as otherCYP2A genes, CYP2A7P1,CYP2B6, CYP2F1, andCYP2GP2 were not detected. These results indicate thatCYP2A7P2 is located near CYP2B7 in the middle of the CYP2A-2B-2F gene cluster on chromosome 19. Furthermore, an analysis of CYP2A sequence alignment suggests thatCYP2A7P2 may be derived from the same ancestral gene that gave rise to CYP2A7P1, which was corrupted by a large insertion at intron 5.
Footnotes
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Send reprint requests to: Dr. Xinxin Ding, Wadsworth Center, New York State Department of Health, Empire State Plaza, Box 509, Albany, NY 12201-0509. E-mail: xding{at}wadsworth.org
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This work was supported in part by Public Health Service Grants DC-02640 and ES-07462 from the National Institutes of Health.
- Abbreviations used are::
- CYP
- cytochrome P450
- kbp
- kilo base pair
- PCR
- polymerase chain reaction
- Received August 31, 2000.
- Accepted October 5, 2000.
- The American Society for Pharmacology and Experimental Therapeutics
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Identification of a New Human CYP2A Gene Fragment with Close Linkage to CYP2GP1
