Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Drug Metabolism & Disposition
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Drug Metabolism & Disposition

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit dmd on Facebook
  • Follow dmd on Twitter
  • Follow ASPET on LinkedIn
Research ArticleArticle

Boron Neutron Capture Therapy of Brain Tumors: Investigation of Urinary Metabolites and Oxidation Products of Sodium Borocaptate by Electrospray Ionization Mass Spectrometry

Christopher R. Gibson, Alfred E. Staubus, Rolf F. Barth, Weilian Yang, Nan M. Kleinholz, R. Benjamin Jones, Kari Green-Church, Werner Tjarks and Albert H. Soloway
Drug Metabolism and Disposition December 2001, 29 (12) 1588-1598;
Christopher R. Gibson
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Alfred E. Staubus
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rolf F. Barth
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Weilian Yang
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nan M. Kleinholz
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
R. Benjamin Jones
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kari Green-Church
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Werner Tjarks
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Albert H. Soloway
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Boron neutron capture therapy (BNCT) is based on a nuclear capture reaction that occurs when boron-10, a stable isotope, is irradiated with low energy neutrons to produce high-energy alpha particles and recoiling lithium-7 nuclei. The purpose of the present study was to determine what urinary metabolites, if any, could be detected in patients with brain tumors who were given sodium borocaptate (BSH), a drug that has been used clinically for BNCT. BSH was infused intravenously over a 1-h time period at doses of 26.5, 44.1, or 88.2 mg/kg of body weight to patients with high-grade brain tumors. Electrospray ionization mass spectrometry has been used to investigate possible urinary metabolites of BSH. Chemical and instrument conditions were established to detect BSH and its possible metabolites in both positive and negative electrospray ionization modes. Using this methodology, boronated ions were found in patients' urine samples that appeared to be consistent with the following chemical structures: BSH sulfenic acid (BSOH), BSH sulfinic acid (BSO2H), BSH disulfide (BSSB), BSH thiosulfinate (BSOSB), and a BSH-S-cysteine conjugate (BSH-CYS). Although BSH has been used clinically for BNCT since the late 1960s, this is the first report of specific biotransformation products following administration to patients. Further studies will be required to determine both the biological significance of these metabolites and whether any of these accumulate in significant amounts in brain tumors.

Footnotes

  • ↵1 Current address: Department of Drug Metabolism, Merck & Co., Inc., WP75A-203, P.O. Box 4, West Point, PA 19486.

  • This work was partially supported by Grant DE-FG02-95EF62059 from the United States Department of Energy. The mass spectrometers were purchased by a grant from the Hayes Investment Fund of the Ohio Board of Regents.

  • Abbreviations used are::
    BNCT
    boron neutron capture therapy
    BSH
    sodium borocaptate
    ESI-MS
    electrospray ionization mass spectrometry
    BSOH
    BSH sulfenic acid
    BSO2H
    BSH sulfinic acid
    BSSB
    BSH disulfide
    BSOSB
    BSH thiosulfinate
    BSH-CYS
    BSH-S-cysteine conjugate
    TBA
    tetrabutylammonium
    TPA
    tetrapentylammonium
    TOF
    time of flight
    MS-MS
    tandem mass spectrometry
    CID
    collision-induced disassociation
    • Received July 17, 2001.
    • Accepted September 14, 2001.
  • The American Society for Pharmacology and Experimental Therapeutics
View Full Text

 

DMD articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Drug Metabolism and Disposition: 29 (12)
Drug Metabolism and Disposition
Vol. 29, Issue 12
1 Dec 2001
  • Table of Contents
  • About the Cover
  • Index by author
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Drug Metabolism & Disposition article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Boron Neutron Capture Therapy of Brain Tumors: Investigation of Urinary Metabolites and Oxidation Products of Sodium Borocaptate by Electrospray Ionization Mass Spectrometry
(Your Name) has forwarded a page to you from Drug Metabolism & Disposition
(Your Name) thought you would be interested in this article in Drug Metabolism & Disposition.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Boron Neutron Capture Therapy of Brain Tumors: Investigation of Urinary Metabolites and Oxidation Products of Sodium Borocaptate by Electrospray Ionization Mass Spectrometry

Christopher R. Gibson, Alfred E. Staubus, Rolf F. Barth, Weilian Yang, Nan M. Kleinholz, R. Benjamin Jones, Kari Green-Church, Werner Tjarks and Albert H. Soloway
Drug Metabolism and Disposition December 1, 2001, 29 (12) 1588-1598;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Boron Neutron Capture Therapy of Brain Tumors: Investigation of Urinary Metabolites and Oxidation Products of Sodium Borocaptate by Electrospray Ionization Mass Spectrometry

Christopher R. Gibson, Alfred E. Staubus, Rolf F. Barth, Weilian Yang, Nan M. Kleinholz, R. Benjamin Jones, Kari Green-Church, Werner Tjarks and Albert H. Soloway
Drug Metabolism and Disposition December 1, 2001, 29 (12) 1588-1598;
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Human ADME Properties of Abrocitinib
  • MSCs Pharmacokinetics under liver diseases
  • OATP1B Downregulation in CDCA-Treated Monkeys
Show more Article

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About DMD
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Journal of Pharmacology and Experimental Therapeutics
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-009X (Online)

Copyright © 2022 by the American Society for Pharmacology and Experimental Therapeutics