Abstract
This study evaluated the toxicokinetics of [14C]di-n-butylphthalate ([14C]DBP) after an intravenous administration (1 and 10 mg/kg, in Cremophor) or a topical application (10 μl/cm2; 10 cm2, neat) in haired male Sprague-Dawley rats. Additional in vivo and in vitro percutaneous penetration studies of [14C]DBP were conducted on male and female haired rats and male hairless rats. After intravenous administration, unchanged DBP disappeared rapidly from the plasma, following a two-exponential function (T1/2β = 5–7 min). The peak levels of monobutylphthalate (MBP) and its glucuronide conjugate (MBP-Gluc) occurred 1 to 2 and 20 to 30 min after administration, respectively. These metabolites were intensively and rapidly excreted in urine (57% of the dose). However, about 35% of the dose recovered in urine was primarily excreted in bile (mainly as MBP-Gluc) and underwent hepatobiliary recycling. Unchanged DBP was barely detectable in excreta. DBP rapidly penetrated the skin, which constituted a reservoir. The absorption flux determined for 0.5 to 8 and 8 to 48 h of exposure were 43 and 156 μg/cm2/h, respectively. The higher flux may be due to radial diffusion of DBP in the stratum and/or epidermis. The in vivo and in vitro experiments revealed that DBP was intensively metabolized into the skin. In vivo percutaneous absorption flux was very similar in male and female haired rats. In contrast, the percutaneous absorption determined in vivo and in vitro was higher in hairless than in haired male rats. Absorption flux was accurately estimated from urinary excretion rate of MBP or MBP-Gluc.
Footnotes
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Send reprint requests to: Dr. Jean-Paul Payan, Institut National de Recherche et de Sécurité, Avenue de Bourgogne, B.P. N° 27, 54501 Vandoeuvre Cedex, France. E-mail:payanjp{at}inrs.fr
- Abbreviations used are::
- DBP
- di-n-butylphthalate
- [14C]DBP
- [14C]di-n-butylphthalate
- MBP
- monobutylphthalate
- MBP-Gluc
- glucuronide conjugate of MBP
- DIPFP
- di-isopropylfluorophosphate
- HPLC
- high-performance liquid chromatography
- AUC
- area under curve
- AUC0–inf (i.v.)
- area under the plasma time curve to infinity, slope of the terminal phase
- CL
- clearance
- Cp
- extrapolated plasma concentration at time T = 0
- Kel
- total elimination rate constant in the central compartment
- MRT
- mean residence time
- % Qo
- percentage of the administered dose
- Tmax
- time for maximal plasma concentration
- Vc
- volume of the central compartment
- Received October 16, 2000.
- Accepted February 8, 2001.
- The American Society for Pharmacology and Experimental Therapeutics
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