Abstract
We found nucleotide variability in the 5′-upstream region and exonic sequences of a gene-encoding canalicular multispecific organic anion transporter/multidrug resistance-associated protein 2 (cMOAT/MRP2) by polymerase chain reaction-based sequencing using genomic DNA from 72 established cell lines derived from 72 Japanese individuals. Four single nucleotide polymorphisms (SNPs) were found in the 5′-untranslational region and 21 in the exonic regions. Of them, 14 were nonsynonymous SNPs. One deletion of seven consecutive adenines resulting in a frameshift variant was also found. Four SNPs, c-24t, g1249a (V417I), c2366t (S789F), and c3972t (I1324I), were the same as those recently reported. A strong association was found between c-24t (5′-untranslated region) and c3972t (exon 28), with the promoter activity of the former worth being compared.
Footnotes
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This study was supported in part by the Program for Promotion of Fundamental Studies in Health Sciences (MPJ-6) of the Organization for Pharmaceutical Safety and Research of Japan.
- Abbreviations used are::
- SNP
- single nucleotide polymorphism
- TM
- transmembrane domain
- cMOAT/MRP2
- canalicular multispecific organic anion transporter/multidrug resistance-associated protein 2
- Received July 31, 2001.
- Accepted December 17, 2001.
- The American Society for Pharmacology and Experimental Therapeutics
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