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Research ArticleArticle

Expression of the Rat CYP2A3 Gene in Transgenic Mice

Ting Su, Qing-Yu Zhang, Jianhua Zhang, Pam Swiatek and Xinxin Ding
Drug Metabolism and Disposition May 2002, 30 (5) 548-552; DOI: https://doi.org/10.1124/dmd.30.5.548
Ting Su
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Qing-Yu Zhang
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Jianhua Zhang
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Pam Swiatek
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Xinxin Ding
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Abstract

Rat CYP2A3 and mouse CYP2A5 are predominantly expressed in the olfactory mucosa. CYP2A3 is also expressed in the lung at a low level, whereas CYP2A5 is expressed in several additional tissues. To better understand the transcriptional regulation of the CYP2Agenes, transgenic mice were generated with a full-lengthCYP2A3 gene fragment containing 3.4 kilobases of the 5′-flanking region. CYP2A3 mRNA was detected in the brain and olfactory bulb in four transgenic mouse lines, in the olfactory mucosa in three lines, and in kidney, liver, lung, and small intestine in two lines. Thus, the expression of the CYP2A3 transgene mimicked the tissue distribution pattern of mouse CYP2A5 rather than that of rat CYP2A3. Furthermore, the levels of CYP2A3 mRNA were very low in all lines examined, suggesting that more distal regulatory regions may be involved in the abundant expression of the CYP2A genes in the olfactory mucosa.

Footnotes

  • ↵1 Current address: Van Andel Research Institute, 333 Bostwick NE, Grand Rapids, MI 49503.

  • This research was supported in part by National Institute of Environmental Health Sciences Grant ES-07462 (National Institutes of Health, Bethesda, MD).

  • Abbreviations used are::
    kb
    kilobase
    bp
    base pair
    PCR
    polymerase chain reaction
    RT
    reverse transcription
    UTR
    untranslated region
    Tm
    melting temperature
    NPTA
    nasal predominant transcriptional activating
    • Received December 12, 2001.
    • Accepted February 21, 2002.
  • The American Society for Pharmacology and Experimental Therapeutics
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Drug Metabolism and Disposition: 30 (5)
Drug Metabolism and Disposition
Vol. 30, Issue 5
1 May 2002
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Research ArticleArticle

Expression of the Rat CYP2A3 Gene in Transgenic Mice

Ting Su, Qing-Yu Zhang, Jianhua Zhang, Pam Swiatek and Xinxin Ding
Drug Metabolism and Disposition May 1, 2002, 30 (5) 548-552; DOI: https://doi.org/10.1124/dmd.30.5.548

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Research ArticleArticle

Expression of the Rat CYP2A3 Gene in Transgenic Mice

Ting Su, Qing-Yu Zhang, Jianhua Zhang, Pam Swiatek and Xinxin Ding
Drug Metabolism and Disposition May 1, 2002, 30 (5) 548-552; DOI: https://doi.org/10.1124/dmd.30.5.548
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