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Research ArticleArticle

SELECTIVE INHIBITION OF HUMAN CYTOCHROME P4502C8 BY MONTELUKAST

Robert L. Walsky, R. Scott Obach, Emily A. Gaman, Jean-Paul R. Gleeson and William R. Proctor
Drug Metabolism and Disposition March 2005, 33 (3) 413-418; DOI: https://doi.org/10.1124/dmd.104.002766
Robert L. Walsky
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R. Scott Obach
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Emily A. Gaman
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Jean-Paul R. Gleeson
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William R. Proctor
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Abstract

The leukotriene receptor antagonist montelukast was examined for its inhibition of the human drug-metabolizing enzyme cytochrome P4502C8 (CYP2C8). Montelukast was demonstrated to be a potent inhibitor of CYP2C8-catalyzed amodiaquine N-deethylase, rosiglitazone N-demethylase, and paclitaxel 6α-hydroxylase in human liver microsomes. Inhibition was also observed when the reaction was catalyzed by recombinant heterologously expressed CYP2C8. The mechanism of inhibition was competitive, with Ki values ranging from 0.0092 to 0.15 μM. Inhibition potency was highly dependent on the microsomal protein concentration. Increasing the microsomal protein concentration by 80-fold yielded a 100-fold decrease in inhibition potency. Preincubation of montelukast with human liver microsomes and NADPH did not alter the inhibition potency, suggesting that montelukast is not a mechanism-based inactivator. Montelukast was a selective inhibitor for human CYP2C8; inhibition of other human cytochrome P450 enzymes was substantially less. These in vitro data support the use of montelukast as a selective CYP2C8 inhibitor that could be used to determine the contribution of this enzyme to drug metabolism reactions. These data also raise the possibility that montelukast could have an effect on the metabolic clearance of drugs possessing CYP2C8-catalyzed metabolism as a major clearance pathway, thereby eliciting pharmacokinetic drug-drug interactions.

Footnotes

  • Article, publication date, and citation information can be found at http://dmd.aspetjournals.org.

  • doi:10.1124/dmd.104.002766.

  • ABBREVIATIONS: P450, cytochrome P450; HPLC, high-performance liquid chromatography; rh, recombinant human.

    • Received October 26, 2004.
    • Accepted December 16, 2004.
  • The American Society for Pharmacology and Experimental Therapeutics
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Drug Metabolism and Disposition: 33 (3)
Drug Metabolism and Disposition
Vol. 33, Issue 3
1 Mar 2005
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Research ArticleArticle

SELECTIVE INHIBITION OF HUMAN CYTOCHROME P4502C8 BY MONTELUKAST

Robert L. Walsky, R. Scott Obach, Emily A. Gaman, Jean-Paul R. Gleeson and William R. Proctor
Drug Metabolism and Disposition March 1, 2005, 33 (3) 413-418; DOI: https://doi.org/10.1124/dmd.104.002766

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Research ArticleArticle

SELECTIVE INHIBITION OF HUMAN CYTOCHROME P4502C8 BY MONTELUKAST

Robert L. Walsky, R. Scott Obach, Emily A. Gaman, Jean-Paul R. Gleeson and William R. Proctor
Drug Metabolism and Disposition March 1, 2005, 33 (3) 413-418; DOI: https://doi.org/10.1124/dmd.104.002766
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