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Research ArticleArticle

Flavonoids Modulate Monocarboxylate Transporter-1-Mediated Transport of γ-Hydroxybutyrate in Vitro and in Vivo

Qi Wang and Marilyn E. Morris
Drug Metabolism and Disposition February 2007, 35 (2) 201-208; DOI: https://doi.org/10.1124/dmd.106.012369
Qi Wang
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Marilyn E. Morris
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Abstract

The objective of this study was to determine the effects of flavonoids on the in vitro monocarboxylate transporter 1 (MCT1)-mediated transport and in vivo disposition of the drug of abuse, γ-hydroxybutyrate (GHB). The uptake of GHB in rat MCT1 gene-transfected MDA-MB231 cells was significantly decreased in the presence of the flavonoids apigenin, biochanin A, chrysin, diosemin, fisetin, genistein, hesperitin, kaempferol, luteolin, morin, narigenin, phloretin, and quercetin, but was not affected by the flavonoid glycosides phloridzin and rutin. The IC50 values for luteolin, morin, and phloretin were 0.41 ± 0.14, 6.41 ± 2.01, and 2.57 ± 0.48 μM, with the inhibition mechanism for luteolin being competitive. [3H]Kaempferol and [3H]biochanin A did not exhibit MCT1-mediated uptake, suggesting that these flavonoids are not substrates for MCT1. The combination of luteolin and phloretin inhibited the uptake of GHB in a synergistic manner; however, the combination of luteolin and morin was antagonistic. GHB 1000 mg/kg was administered to rats by i.v. bolus, with or without the concomitant administration of luteolin 10 mg/kg i.v. After luteolin treatment, the renal and total clearances of GHB were significantly increased, probably because of inhibition of the MCT1-mediated renal reabsorption of GHB, and the sleep time significantly decreased (121 ± 5 min versus 165 ± 10 min) compared with control rats. Overall, the results of this study indicate that flavonoids from food or herbal products may significantly alter the pharmacokinetics and pharmacodynamics of MCT substrates.

Footnotes

  • Financial support was provided by National Institutes of Health Grant DA14988.

  • Article, publication date, and citation information can be found at http://dmd.aspetjournals.org.

  • doi:10.1124/dmd.106.012369.

  • ABBREVIATIONS: GHB, γ-hydroxybutyrate; MCT, monocarboxylate transporter; CHC, α-cyano-4-hydroxycinnamic acid; ABC, ATP-binding cassette; MRP, multidrug resistance-associated protein; OATP, organic anion-transporting protein; PK, pharmacokinetics; LC/MS/MS, liquid chromatography-tandem mass spectrometry; ANOVA, analysis of variance; AUC, area under the curve; CI, combination index.

    • Received August 3, 2006.
    • Accepted November 8, 2006.
  • The American Society for Pharmacology and Experimental Therapeutics
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Drug Metabolism and Disposition: 35 (2)
Drug Metabolism and Disposition
Vol. 35, Issue 2
1 Feb 2007
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Research ArticleArticle

Flavonoids Modulate Monocarboxylate Transporter-1-Mediated Transport of γ-Hydroxybutyrate in Vitro and in Vivo

Qi Wang and Marilyn E. Morris
Drug Metabolism and Disposition February 1, 2007, 35 (2) 201-208; DOI: https://doi.org/10.1124/dmd.106.012369

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Research ArticleArticle

Flavonoids Modulate Monocarboxylate Transporter-1-Mediated Transport of γ-Hydroxybutyrate in Vitro and in Vivo

Qi Wang and Marilyn E. Morris
Drug Metabolism and Disposition February 1, 2007, 35 (2) 201-208; DOI: https://doi.org/10.1124/dmd.106.012369
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