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Research ArticleArticle

Comparison of Two Immortalized Human Cell Lines to Study Nuclear Receptor-Mediated CYP3A4 Induction

S. Harmsen, A. S. Koster, J. H. Beijnen, J. H. M. Schellens and I. Meijerman
Drug Metabolism and Disposition June 2008, 36 (6) 1166-1171; DOI: https://doi.org/10.1124/dmd.107.017335
S. Harmsen
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A. S. Koster
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J. H. Beijnen
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J. H. M. Schellens
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I. Meijerman
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Abstract

Since CYP3A4 is responsible for the biotransformation of over 50% of all clinically used drugs, induction results in an increased clearance of many concomitantly administered drugs, thereby decreasing treatment efficacy or, in the case of prodrugs, lead to severe intoxications. CYP3A4 induction is regulated by the pregnane X receptor, constitutive androstane receptor, and vitamin D receptor. Since these nuclear receptors show large interspecies differences, accurate prediction of nuclear receptor-mediated CYP3A4 induction in humans requires the use of human systems. Because primary cultures of human hepatocytes or enterocytes have major drawbacks like poor availability and poor reproducibility, human cell lines are a good alternative. In this study, the widely used HepG2 cell line was compared with the LS180 cell line to serve as a model to study CYP3A4 induction. There was a clear difference between the cell lines with respect to CYP3A enzyme expression and induction. In LS180, CYP3A4 was expressed and was found to be induced by prototypical nuclear receptor agonists, whereas in HepG2, CYP3A4 was nonresponsive to treatment with rifampicin, CITCO [6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde-O-3,4-dichlorobenzyl) oxime], or calcitriol. We subsequently evaluated whether these host-cell differences also have an effect on CYP3A4 reporter gene activity. We clearly show that there are differences in CYP3A4 reporter activity between the cell lines, and based on these results and those found on mRNA and protein level, we conclude that LS180 is a more suitable cell line to study CYP3A4 induction than the widely used HepG2.

Footnotes

  • This study was supported by the Maurits and Anna de Kock Foundation and by the Nijbakker-Morra Foundation.

  • Article, publication date, and citation information can be found at http://dmd.aspetjournals.org.

  • doi:10.1124/dmd.107.017335.

  • ABBREVIATIONS: PXR, pregnane X receptor; NR, nuclear receptor; CAR, constitutive androstane receptor; VDR, vitamin D3 receptor; CITCO, 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde-O-3,4-dichlorobenzyl) oxime; PBS, phosphate-buffered saline; PCR, polymerase chain reaction; DMSO, dimethylsulfoxide.

    • Received June 21, 2007.
    • Accepted March 14, 2008.
  • The American Society for Pharmacology and Experimental Therapeutics
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Drug Metabolism and Disposition: 36 (6)
Drug Metabolism and Disposition
Vol. 36, Issue 6
1 Jun 2008
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Research ArticleArticle

Comparison of Two Immortalized Human Cell Lines to Study Nuclear Receptor-Mediated CYP3A4 Induction

S. Harmsen, A. S. Koster, J. H. Beijnen, J. H. M. Schellens and I. Meijerman
Drug Metabolism and Disposition June 1, 2008, 36 (6) 1166-1171; DOI: https://doi.org/10.1124/dmd.107.017335

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Research ArticleArticle

Comparison of Two Immortalized Human Cell Lines to Study Nuclear Receptor-Mediated CYP3A4 Induction

S. Harmsen, A. S. Koster, J. H. Beijnen, J. H. M. Schellens and I. Meijerman
Drug Metabolism and Disposition June 1, 2008, 36 (6) 1166-1171; DOI: https://doi.org/10.1124/dmd.107.017335
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