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Research ArticleArticle

Metabolism of Sesamin by Cytochrome P450 in Human Liver Microsomes

Kaori Yasuda, Shinichi Ikushiro, Masaki Kamakura, Miho Ohta and Toshiyuki Sakaki
Drug Metabolism and Disposition December 2010, 38 (12) 2117-2123; DOI: https://doi.org/10.1124/dmd.110.035659
Kaori Yasuda
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Shinichi Ikushiro
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Masaki Kamakura
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Miho Ohta
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Toshiyuki Sakaki
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Abstract

Metabolism of sesamin by cytochrome P450 (P450) was examined using yeast expression system and human liver microsomes. Saccharomyces cerevisiae cells expressing each of human P450 isoforms (CYP1A1, 1A2, 2A6, 2B6, 2C8, 2C9, 2C18, 2C19, 2D6, 2E1, and 3A4) were cultivated with sesamin, and monocatechol metabolite was observed in most of P450s. Kinetic analysis using the microsomal fractions of the recombinant S. cerevisiae cells revealed that CYP2C19 had the largest kcat/Km value. Based on the kinetic data and average contents of the P450 isoforms in the human liver, the putative contribution of P450s for sesamin metabolism was large in the order of CYP2C9, 1A2, 2C19, and 2D6. A good correlation was observed between sesamin catecholization activity and CYP2C9-specific activity in in vitro studies using 10 individual human liver microsomes, strongly suggesting that CYP2C9 is the most important P450 isoform for sesamin catecholization in human liver. Inhibition studies using each anti-P450 isoform-specific antibody confirmed that CYP2C9 was the most important, and the secondary most important P450 was CYP1A2. We also examined the inhibitory effect of sesamin for P450 isoform-specific activities and found a mechanism-based inhibition of CYP2C9 by sesamin. In contrast, no mechanism-based inhibition by sesamin was observed in CYP1A2-specific activity. Our findings strongly suggest that further studies are needed to reveal the interaction between sesamin and therapeutic drugs mainly metabolized by CYP2C9.

Footnotes

  • This work was supported in part by a Ministry of Education, Culture, Sports, Science and Technology grant.

  • Article, publication date, and citation information can be found at http://dmd.aspetjournals.org.

    doi:10.1124/dmd.110.035659.

  • ABBREVIATIONS:

    P450
    cytochrome P450
    MBI
    mechanism-based inhibition
    MDP
    methylenedioxyphenyl
    DMSO
    dimethyl sulfoxide
    HPLC
    high-performance liquid chromatography.

  • Received July 25, 2010.
  • Accepted September 17, 2010.
  • Copyright © 2010 by The American Society for Pharmacology and Experimental Therapeutics
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Drug Metabolism and Disposition: 38 (12)
Drug Metabolism and Disposition
Vol. 38, Issue 12
1 Dec 2010
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Research ArticleArticle

Metabolism of Sesamin by Cytochrome P450 in Human Liver Microsomes

Kaori Yasuda, Shinichi Ikushiro, Masaki Kamakura, Miho Ohta and Toshiyuki Sakaki
Drug Metabolism and Disposition December 1, 2010, 38 (12) 2117-2123; DOI: https://doi.org/10.1124/dmd.110.035659

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Research ArticleArticle

Metabolism of Sesamin by Cytochrome P450 in Human Liver Microsomes

Kaori Yasuda, Shinichi Ikushiro, Masaki Kamakura, Miho Ohta and Toshiyuki Sakaki
Drug Metabolism and Disposition December 1, 2010, 38 (12) 2117-2123; DOI: https://doi.org/10.1124/dmd.110.035659
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