Abstract
Kakkalide is a major isoflavonoid from the flowers of Pueraria lobata (Willd.) Ohwi, possessing the protective effect against ethanol-induced intoxication and hepatic injury. The metabolism of kakkalide was investigated in rats. Thirteen metabolites were isolated by using solvent extraction and repeated chromatographic methods and identified by using spectroscopic methods including UV, IR, mass spectrometry, NMR, and circular dichroism experiments. Four new compounds were identified as irisolidone-7-O-glucuronide (M-1), tectorigenin-7-O-sulfate (M-2), tectorigenin-4′-O-sulfate (M-3), and biochanin A-6-O-sulfate (M-4) together with nine known compounds identified as irisolidone (M-5), tectorigenin (M-6), tectoridin (M-7), 5,7-dihydroxy-8,4′-dimethoxyisoflavone (M-8), isotectorigenin (M-9), biochanin A (M-10), genistein (M-11), daidzein (M-12), and equol (M-13). The metabolic pathway of kakkalide was proposed, which is important to understand its metabolic fate and disposition in humans.
Footnotes
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This work was supported by the Research Fund for the Doctoral Program of Higher Education from the Ministry of Education of the People's Republic of China.
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Article, publication date, and citation information can be found at http://dmd.aspetjournals.org.
doi:10.1124/dmd.109.028555
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- LC-MSn
- liquid chromatography-tandem mass spectrometry
- MS
- mass spectrometry
- HPLC
- high-performance liquid chromatography
- CC
- column chromatography
- CD
- circular dichroism
- ESI
- electrospray ionization
- TOF-MS
- time-of-flight tandem mass spectrometry.
- Received June 2, 2009.
- Accepted November 5, 2009.
- Copyright © 2010 by The American Society for Pharmacology and Experimental Therapeutics
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