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Research ArticleArticle

Use of the Entero-Test, a Novel Approach for the Noninvasive Capture of Biliary Metabolites in Dogs

W. J. Guiney, C. Beaumont and S. R. Thomas
Drug Metabolism and Disposition May 2010, 38 (5) 851-856; DOI: https://doi.org/10.1124/dmd.109.031062
W. J. Guiney
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C. Beaumont
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S. R. Thomas
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Abstract

Preclinical information on the biliary metabolites of a drug candidate is typically obtained through the collection of bile after surgical cannulation of the bile duct. In this study, we describe a novel approach using the Entero-Test, a simple device that facilitates the noninvasive sampling of duodenal bile. The Entero-Test was used to collect bile from six fasted dogs that had been dosed either orally with simvastatin (SV) or intravenously with simvastatin hydroxy acid (SVA), compounds that have been previously reported to undergo extensive metabolism and biliary secretion in the dog. The devices, consisting of a weighted gelatin capsule containing 90 cm of a highly absorbent nylon string, were swallowed by each dog with the proximal end of the string taped to the animal’s face. Once the weighted string had reached the duodenum, gallbladder contraction was stimulated to release bile. Each bile-stained string was then retrieved via the mouth and, after solvent extraction, samples were analyzed for drug-related material by ultraperformance liquid chromatography-mass spectrometry and NMR spectroscopy. Numerous metabolites of SV and SVA were observed, and, in general, the major metabolites have been reported previously from studies with bile duct-cannulated animals dosed with [14C]SV or [14C]SVA. The results from this study demonstrate the utility of deploying the Entero-Test in absorption, distribution, metabolism, and elimination studies to provide information on the nature of biliary metabolites, which, on occasion, may be sufficient to negate the need for more invasive sampling techniques. The benefits and limitations of the technique are discussed.

Footnotes

  • Article, publication date, and citation information can be found at http://dmd.aspetjournals.org.

    doi:10.1124/dmd.109.031062.

  • ADME
    absorption, distribution, metabolism and elimination
    SV
    simvastatin
    SVA
    simvastatin hydroxy acid
    HPLC
    high-performance liquid chromatography
    UPLC
    ultraperformance liquid chromatography
    MS
    mass spectrometry
    CCK
    cholecystokinin.

    • Received November 6, 2009.
    • Accepted January 28, 2010.
  • Copyright © 2010 by The American Society for Pharmacology and Experimental Therapeutics
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Drug Metabolism and Disposition: 38 (5)
Drug Metabolism and Disposition
Vol. 38, Issue 5
1 May 2010
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Research ArticleArticle

Use of the Entero-Test, a Novel Approach for the Noninvasive Capture of Biliary Metabolites in Dogs

W. J. Guiney, C. Beaumont and S. R. Thomas
Drug Metabolism and Disposition May 1, 2010, 38 (5) 851-856; DOI: https://doi.org/10.1124/dmd.109.031062

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Research ArticleArticle

Use of the Entero-Test, a Novel Approach for the Noninvasive Capture of Biliary Metabolites in Dogs

W. J. Guiney, C. Beaumont and S. R. Thomas
Drug Metabolism and Disposition May 1, 2010, 38 (5) 851-856; DOI: https://doi.org/10.1124/dmd.109.031062
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