Abstract
Drug pharmacokinetics can be altered in obese and diabetic subjects. In consideration of the prevalence of obesity and diabetes, characterization of transporter expression in mouse models of diabetes and obesity may be a useful tool to aid in prediction of altered drug pharmacokinetics or adverse drug reactions. It has been reported that ob/ob mice, which display a severe obesity and diabetes phenotype, exhibit multiple changes in drug transporter expression in liver and kidney. In the present study, the mRNA and protein expression of major drug transporters was determined in livers and kidneys of diet-induced obese (DIO) C57BL/6J male mice. The mice were fed a high-fat diet (HFD) (60% fat) from 6 weeks of age and display obesity, fatty liver, and mild hyperglycemia. The HFD diet increased expression of multidrug resistance-associated proteins Abcc3 and 4 mRNA and protein in liver by 3.4- and 1.4-fold, respectively, compared with that detected in control mice fed a low-fat diet (LFD). In contrast, Abcc1 mRNA and protein decreased by 50% in livers of DIO mice compared with those in livers to lean mice. The HFD did not alter transporter expression in kidney compared with the LFD. In summary, unlike ob/ob and db/db mice, DIO mice exhibited a selective induction of efflux transporter expression in liver (i.e., Abcc3 and 4). In addition, diet-induced obesity affects transporter expression in liver but not kidney in the C57BL/6J mouse model. These data indicate that hepatic transporter expression is only slightly altered in a model of mild diabetes and nonalcoholic fatty liver disease and obesity.
Footnotes
This work was supported by the National Institutes of Health National Institute of Environmental Health Sciences [Grants 1R01-ES016042, 5K22-ES013782]; the National Institutes of Health National Center for Research Resources [Grant P20-RR016457-10] (Rhode Island IDeA Network of Biomedical Research Excellence); and the Rhode Island Foundation.
Article, publication date, and citation information can be found at http://dmd.aspetjournals.org.
doi:10.1124/dmd.110.037507.
↵ The online version of this article (available at http://dmd.aspetjournals.org) contains supplemental material.
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ABBREVIATIONS:
- BMI
- body mass index
- FFA
- free fatty acid
- NAFLD
- nonalcoholic fatty liver disease
- NASH
- nonalcoholic steatohepatitis
- DIO
- diet-induced obesity
- SLC/Slc
- solute carrier superfamily
- ABC/Abc
- ATP-binding cassette
- oat
- organic anion-transporting polypeptide
- oct
- organic cation transporter
- PVDF
- polyvinylidene difluoride
- Mcp-1
- monocyte chemoattractant protein-1
- Icam-1
- intracellular adhesion molecule-1
- ATV
- atorvastatin
- CAR
- constitutive androstane receptor
- Nrf2
- nuclear factor E2-related factor 2.
- Received November 30, 2010.
- Accepted March 22, 2011.
- Copyright © 2011 by The American Society for Pharmacology and Experimental Therapeutics
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