Parts of this work were previously presented at the following meetings: Parkinson A (2009) Metabolism-dependent inhibition (MDI) studies: ten things worth knowing and Paris BL, Kazmi F, Buckley DB, Ogilvie BW, Gipson AE, and Parkinson A (2009) Pitfalls in the design of CYP inhibition studies incorporating a dilution step to examine time-dependent inhibition (TDI) or metabolism-dependent inhibition (MDI). Sixteenth North American Regional ISSX Meeting; 2009 Oct 18–22; Baltimore, MD. International Society for the Study of Xenobiotics, Washington, DC; Buckley DB, Kazmi F, Yerino P, Toren P, Holsapple J, Ogilvie BW, Paris BL, and Parkinson A (2010) Pitfalls in the design of metabolism-dependent CYP inhibition (MDI) experiments with a dilution step: inhibitor depletion by metabolism and/or microsomal binding leads to underestimation of the shifted IC₅₀ value. Ninth International ISSX Meeting; 2010 Sept 4–8; Istanbul, Turkey. International Society for the Study of Xenobiotics, Washington, DC.