Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Drug Metabolism & Disposition
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Drug Metabolism & Disposition

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit dmd on Facebook
  • Follow dmd on Twitter
  • Follow ASPET on LinkedIn
Research ArticleArticle

Xenobiotic Metabolism and Disposition in Human Lung Cell Models: Comparison with In Vivo Expression Profiles

Elisabeth Courcot, Julie Leclerc, Jean-Jacques Lafitte, Eric Mensier, Sophie Jaillard, Philippe Gosset, Pirouz Shirali, Nicolas Pottier, Franck Broly and Jean-Marc Lo-Guidice
Drug Metabolism and Disposition October 2012, 40 (10) 1953-1965; DOI: https://doi.org/10.1124/dmd.112.046896
Elisabeth Courcot
Faculté de Médecine H. Warembourg, Pôle Recherche, Lille, France (E.C., F.B., J.L., J.-M.L.-G., N.P.); Service de Pneumologie et d'Oncologie Thoracique, Hôpital Calmette, Centre Hospitalier Régional Universitaire, Lille, France (J.-J.L.); Département de Chirurgie, Polyclinique du Bois, Lille, France (E.M., S.J.); Unité de Chimie Environnementale et Interactions sur le Vivant, Université du Littoral-Côte d'Opale, Dunkerque, France (P.S.); and Unité Institut National de la Santé et de la Recherche Médicale U1019, Institut Pasteur de Lille, Lille, France (P.G.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Julie Leclerc
Faculté de Médecine H. Warembourg, Pôle Recherche, Lille, France (E.C., F.B., J.L., J.-M.L.-G., N.P.); Service de Pneumologie et d'Oncologie Thoracique, Hôpital Calmette, Centre Hospitalier Régional Universitaire, Lille, France (J.-J.L.); Département de Chirurgie, Polyclinique du Bois, Lille, France (E.M., S.J.); Unité de Chimie Environnementale et Interactions sur le Vivant, Université du Littoral-Côte d'Opale, Dunkerque, France (P.S.); and Unité Institut National de la Santé et de la Recherche Médicale U1019, Institut Pasteur de Lille, Lille, France (P.G.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jean-Jacques Lafitte
Faculté de Médecine H. Warembourg, Pôle Recherche, Lille, France (E.C., F.B., J.L., J.-M.L.-G., N.P.); Service de Pneumologie et d'Oncologie Thoracique, Hôpital Calmette, Centre Hospitalier Régional Universitaire, Lille, France (J.-J.L.); Département de Chirurgie, Polyclinique du Bois, Lille, France (E.M., S.J.); Unité de Chimie Environnementale et Interactions sur le Vivant, Université du Littoral-Côte d'Opale, Dunkerque, France (P.S.); and Unité Institut National de la Santé et de la Recherche Médicale U1019, Institut Pasteur de Lille, Lille, France (P.G.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Eric Mensier
Faculté de Médecine H. Warembourg, Pôle Recherche, Lille, France (E.C., F.B., J.L., J.-M.L.-G., N.P.); Service de Pneumologie et d'Oncologie Thoracique, Hôpital Calmette, Centre Hospitalier Régional Universitaire, Lille, France (J.-J.L.); Département de Chirurgie, Polyclinique du Bois, Lille, France (E.M., S.J.); Unité de Chimie Environnementale et Interactions sur le Vivant, Université du Littoral-Côte d'Opale, Dunkerque, France (P.S.); and Unité Institut National de la Santé et de la Recherche Médicale U1019, Institut Pasteur de Lille, Lille, France (P.G.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Sophie Jaillard
Faculté de Médecine H. Warembourg, Pôle Recherche, Lille, France (E.C., F.B., J.L., J.-M.L.-G., N.P.); Service de Pneumologie et d'Oncologie Thoracique, Hôpital Calmette, Centre Hospitalier Régional Universitaire, Lille, France (J.-J.L.); Département de Chirurgie, Polyclinique du Bois, Lille, France (E.M., S.J.); Unité de Chimie Environnementale et Interactions sur le Vivant, Université du Littoral-Côte d'Opale, Dunkerque, France (P.S.); and Unité Institut National de la Santé et de la Recherche Médicale U1019, Institut Pasteur de Lille, Lille, France (P.G.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Philippe Gosset
Faculté de Médecine H. Warembourg, Pôle Recherche, Lille, France (E.C., F.B., J.L., J.-M.L.-G., N.P.); Service de Pneumologie et d'Oncologie Thoracique, Hôpital Calmette, Centre Hospitalier Régional Universitaire, Lille, France (J.-J.L.); Département de Chirurgie, Polyclinique du Bois, Lille, France (E.M., S.J.); Unité de Chimie Environnementale et Interactions sur le Vivant, Université du Littoral-Côte d'Opale, Dunkerque, France (P.S.); and Unité Institut National de la Santé et de la Recherche Médicale U1019, Institut Pasteur de Lille, Lille, France (P.G.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Pirouz Shirali
Faculté de Médecine H. Warembourg, Pôle Recherche, Lille, France (E.C., F.B., J.L., J.-M.L.-G., N.P.); Service de Pneumologie et d'Oncologie Thoracique, Hôpital Calmette, Centre Hospitalier Régional Universitaire, Lille, France (J.-J.L.); Département de Chirurgie, Polyclinique du Bois, Lille, France (E.M., S.J.); Unité de Chimie Environnementale et Interactions sur le Vivant, Université du Littoral-Côte d'Opale, Dunkerque, France (P.S.); and Unité Institut National de la Santé et de la Recherche Médicale U1019, Institut Pasteur de Lille, Lille, France (P.G.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nicolas Pottier
Faculté de Médecine H. Warembourg, Pôle Recherche, Lille, France (E.C., F.B., J.L., J.-M.L.-G., N.P.); Service de Pneumologie et d'Oncologie Thoracique, Hôpital Calmette, Centre Hospitalier Régional Universitaire, Lille, France (J.-J.L.); Département de Chirurgie, Polyclinique du Bois, Lille, France (E.M., S.J.); Unité de Chimie Environnementale et Interactions sur le Vivant, Université du Littoral-Côte d'Opale, Dunkerque, France (P.S.); and Unité Institut National de la Santé et de la Recherche Médicale U1019, Institut Pasteur de Lille, Lille, France (P.G.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Franck Broly
Faculté de Médecine H. Warembourg, Pôle Recherche, Lille, France (E.C., F.B., J.L., J.-M.L.-G., N.P.); Service de Pneumologie et d'Oncologie Thoracique, Hôpital Calmette, Centre Hospitalier Régional Universitaire, Lille, France (J.-J.L.); Département de Chirurgie, Polyclinique du Bois, Lille, France (E.M., S.J.); Unité de Chimie Environnementale et Interactions sur le Vivant, Université du Littoral-Côte d'Opale, Dunkerque, France (P.S.); and Unité Institut National de la Santé et de la Recherche Médicale U1019, Institut Pasteur de Lille, Lille, France (P.G.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Jean-Marc Lo-Guidice
Faculté de Médecine H. Warembourg, Pôle Recherche, Lille, France (E.C., F.B., J.L., J.-M.L.-G., N.P.); Service de Pneumologie et d'Oncologie Thoracique, Hôpital Calmette, Centre Hospitalier Régional Universitaire, Lille, France (J.-J.L.); Département de Chirurgie, Polyclinique du Bois, Lille, France (E.M., S.J.); Unité de Chimie Environnementale et Interactions sur le Vivant, Université du Littoral-Côte d'Opale, Dunkerque, France (P.S.); and Unité Institut National de la Santé et de la Recherche Médicale U1019, Institut Pasteur de Lille, Lille, France (P.G.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Numerous lung cell lines are currently used as in vitro models for pharmacological and toxicological studies. However, no exhaustive report about the metabolic capacities of these models in comparison with those of lung tissues is available. In the present study, we used a high-throughput quantitative real-time reverse transcription-polymerase chain reaction strategy to characterize the expression profiles of 380 genes encoding proteins involved in the metabolism and disposition of xenobiotics in 10 commonly used lung cell lines (A549, H292, H358, H460, H727, Calu-1, 16HBE, 1 HAEO, BEAS-2B, and L-132) and four primary cultures of human bronchial epithelial cells. Expression results were then compared with those previously obtained in human nontumoral and tumoral lung tissues. Our results revealed disparities in gene expression between lung cell lines or when comparing lung cell lines with primary cells or lung tissues. Primary cell cultures displayed the highest similarities with bronchial mucosa in terms of transcript profiling and therefore seem to be the most relevant in vitro model for investigating the metabolism and bioactivation of toxicants and drugs in bronchial epithelium. H292 and BEAS-2B cell lines, which exhibited the highest homology in gene expression pattern with primary cells and the lowest number of dysregulated genes compared with nontumoral lung tissues, could be used as surrogates for toxicological and pharmacological studies. Overall, our study should provide references for researchers to choose the most appropriate in vitro model for analyzing the cellular effects of drugs or airborne toxicants on the airway.

Footnotes

  • This work was supported by the Institut de Recherche en Environnement Industriel; the Université de Lille 2; and the Conseil Régional du Nord-Pas-de-Calais.

  • Article, publication date, and citation information can be found at http://dmd.aspetjournals.org.

    http://dx.doi.org/10.1124/dmd.112.046896.

  • ABBREVIATIONS:

    XME
    xenobiotic-metabolizing enzyme
    P450
    cytochrome P450
    SLC
    solute carriers
    ABC
    ATP-binding cassette
    AhR
    aryl hydrocarbon receptor
    PP
    pulmonary parenchyma
    BM
    bronchial mucosa
    AC
    adenocarcinoma
    RT-PCR
    reverse transcription-polymerase chain reaction
    TLDA
    TaqMan low-density arrays
    SCC
    squamous cell carcinoma
    HBEC
    human bronchial epithelial cell
    Ct
    threshold cycle
    RQ
    relative quantity
    GST
    glutathione transferase
    AIP
    aryl hydrocarbon receptor interacting protein
    ARNT
    AHR nuclear translocator
    HSP
    heat shock protein
    CV
    coefficient of variation
    MRP1
    multidrug resistance protein 1
    OATP
    organic anion-transporting polypeptide
    PPARD
    peroxisome proliferator-activated receptor delta
    RARG
    retinoic acid receptor gamma
    VDR
    vitamine D receptor.

  • Received May 23, 2012.
  • Accepted July 13, 2012.
  • Copyright © 2012 by The American Society for Pharmacology and Experimental Therapeutics
View Full Text

 

DMD articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Drug Metabolism and Disposition: 40 (10)
Drug Metabolism and Disposition
Vol. 40, Issue 10
1 Oct 2012
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Drug Metabolism & Disposition article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Xenobiotic Metabolism and Disposition in Human Lung Cell Models: Comparison with In Vivo Expression Profiles
(Your Name) has forwarded a page to you from Drug Metabolism & Disposition
(Your Name) thought you would be interested in this article in Drug Metabolism & Disposition.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

XENOBIOTIC METABOLISM AND DISPOSITION IN LUNG CELLS MODELS

Elisabeth Courcot, Julie Leclerc, Jean-Jacques Lafitte, Eric Mensier, Sophie Jaillard, Philippe Gosset, Pirouz Shirali, Nicolas Pottier, Franck Broly and Jean-Marc Lo-Guidice
Drug Metabolism and Disposition October 1, 2012, 40 (10) 1953-1965; DOI: https://doi.org/10.1124/dmd.112.046896

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

XENOBIOTIC METABOLISM AND DISPOSITION IN LUNG CELLS MODELS

Elisabeth Courcot, Julie Leclerc, Jean-Jacques Lafitte, Eric Mensier, Sophie Jaillard, Philippe Gosset, Pirouz Shirali, Nicolas Pottier, Franck Broly and Jean-Marc Lo-Guidice
Drug Metabolism and Disposition October 1, 2012, 40 (10) 1953-1965; DOI: https://doi.org/10.1124/dmd.112.046896
del.icio.us logo Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Authorship Contributions
    • Acknowledgments
    • Footnotes
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • Identification of payload-containing catabolites of ADCs
  • PK Interactions of Licorice with Cytochrome P450s
  • Biotransformation of Trastuzumab and Pertuzumab
Show more Articles

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About DMD
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Journal of Pharmacology and Experimental Therapeutics
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-009X (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics