Skip to main content
Advertisement

Main menu

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET

User menu

  • My alerts
  • Log in
  • My Cart

Search

  • Advanced search
Drug Metabolism & Disposition
  • Other Publications
    • Drug Metabolism and Disposition
    • Journal of Pharmacology and Experimental Therapeutics
    • Molecular Pharmacology
    • Pharmacological Reviews
    • Pharmacology Research & Perspectives
    • ASPET
  • My alerts
  • Log in
  • My Cart
Drug Metabolism & Disposition

Advanced Search

  • Home
  • Articles
    • Current Issue
    • Fast Forward
    • Latest Articles
    • Special Sections
    • Archive
  • Information
    • Instructions to Authors
    • Submit a Manuscript
    • FAQs
    • For Subscribers
    • Terms & Conditions of Use
    • Permissions
  • Editorial Board
  • Alerts
    • Alerts
    • RSS Feeds
  • Virtual Issues
  • Feedback
  • Submit
  • Visit dmd on Facebook
  • Follow dmd on Twitter
  • Follow ASPET on LinkedIn
Research ArticleArticle

Nicotine Kinetics in Zebra Finches In Vivo and In Vitro

Sharon Miksys, Susanne L.T. Cappendijk, William M. Perry, Bin Zhao and Rachel F. Tyndale
Drug Metabolism and Disposition June 2013, 41 (6) 1240-1246; DOI: https://doi.org/10.1124/dmd.112.050765
Sharon Miksys
Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Departments of Pharmacology and Toxicology and Psychiatry, University of Toronto, Toronto, Canada (S.M., B.Z., R.F.T.); and Department of Biomedical Sciences, Florida State University, Tallahassee, Florida (S.L.T.C., W.M.P.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Susanne L.T. Cappendijk
Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Departments of Pharmacology and Toxicology and Psychiatry, University of Toronto, Toronto, Canada (S.M., B.Z., R.F.T.); and Department of Biomedical Sciences, Florida State University, Tallahassee, Florida (S.L.T.C., W.M.P.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
William M. Perry
Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Departments of Pharmacology and Toxicology and Psychiatry, University of Toronto, Toronto, Canada (S.M., B.Z., R.F.T.); and Department of Biomedical Sciences, Florida State University, Tallahassee, Florida (S.L.T.C., W.M.P.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Bin Zhao
Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Departments of Pharmacology and Toxicology and Psychiatry, University of Toronto, Toronto, Canada (S.M., B.Z., R.F.T.); and Department of Biomedical Sciences, Florida State University, Tallahassee, Florida (S.L.T.C., W.M.P.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rachel F. Tyndale
Campbell Family Mental Health Research Institute, Centre for Addiction and Mental Health, Departments of Pharmacology and Toxicology and Psychiatry, University of Toronto, Toronto, Canada (S.M., B.Z., R.F.T.); and Department of Biomedical Sciences, Florida State University, Tallahassee, Florida (S.L.T.C., W.M.P.)
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF
Loading

Abstract

Nicotine enhances cognitive performance, and in the zebra finch (Taeniopygia guttata), which is a well-established model of cognition, the effects of nicotine on song production have been reported. Nicotine and cotinine plasma levels were assessed in vivo after subcutaneous injection of 0.18 mg/kg nicotine, a dose that elicits changes in song production. The half-life of nicotine elimination was 33 minutes, and levels were undetectable by 4 hours. Average plasma nicotine over 2 hours was 32 ng/ml, similar to levels seen in human smokers and rat models of nicotine behavior. Nicotine brain levels were 30 and 14 ng/g 1 and 2 hours after treatment. To understand the potential for drug interactions and the regulation of nicotine metabolism in zebra finches, we characterized in vitro nicotine metabolism and the hepatic enzyme involved. In humans, cytochrome P450 2A6 metabolizes nicotine to cotinine, and CYP2A-like activity and protein have been reported in some birds. Zebra finch liver microsomes metabolized nicotine and bupropion (a CYP2B substrate) but not coumarin (a CYP2A substrate). Nicotine was metabolized to cotinine with a Michaelis-Menten constant (Km) of 96 µM and a Vmax of 56 pmol/min per milligram. Nicotine and bupropion metabolism was inhibited by C-8-xanthate (a specific CYP2B inhibitor) but not by CYP2A-specific inhibitors, and hepatic levels of CYP2B-like but not CYP2A-like proteins correlated with nicotine (r = 0.52; P = 0.04) and bupropion metabolism (r = 0.81; P < 0.001), suggesting CYP2B-mediation of nicotine metabolism as seen in rats. These results will facilitate further investigation of nicotine’s effects in zebra finches.

Footnotes

    • Received December 19, 2012.
    • Accepted March 25, 2013.
  • This work was supported by the Canadian Institutes of Health Research [Grants MOP 97751 and MOP86471], a Canada Research Chair; the Campbell Family Mental Health Research Institute Centre for Addiction and Mental Health (CAMH) and the Canada Foundation for Innovation [Grants 20289 and 16014]; the CAMH Foundation and the Ontario Ministry of Research and Innovation and the James and Esther King Biomedical Research Program [Grant 06-NIR02].

  • R.F.T. has participated in one-day advisory meetings for Novartis and McNeil. Parts of this study were presented previously at the following meeting: Miksys S, Zhao B, Lo S, Smith D, Cappendijk SLT, and Tyndale RF (2012) Zebra Finches metabolize nicotine with a CYP2B-like enzyme. Society for Research on Nicotine and Tobacco Annual Meeting; 2012 Mar 13–16; Houston, TX.

  • dx.doi.org/10.1124/dmd.112.050765.

  • Copyright © 2013 by The American Society for Pharmacology and Experimental Therapeutics
View Full Text

 

DMD articles become freely available 12 months after publication, and remain freely available for 5 years. 

Non-open access articles that fall outside this five year window are available only to institutional subscribers and current ASPET members, or through the article purchase feature at the bottom of the page. 

 

  • Click here for information on institutional subscriptions.
  • Click here for information on individual ASPET membership.

 

Log in using your username and password

Forgot your user name or password?

Purchase access

You may purchase access to this article. This will require you to create an account if you don't already have one.
PreviousNext
Back to top

In this issue

Drug Metabolism and Disposition: 41 (6)
Drug Metabolism and Disposition
Vol. 41, Issue 6
1 Jun 2013
  • Table of Contents
  • Table of Contents (PDF)
  • About the Cover
  • Index by author
  • Editorial Board (PDF)
  • Front Matter (PDF)
Download PDF
Article Alerts
Sign In to Email Alerts with your Email Address
Email Article

Thank you for sharing this Drug Metabolism & Disposition article.

NOTE: We request your email address only to inform the recipient that it was you who recommended this article, and that it is not junk mail. We do not retain these email addresses.

Enter multiple addresses on separate lines or separate them with commas.
Nicotine Kinetics in Zebra Finches In Vivo and In Vitro
(Your Name) has forwarded a page to you from Drug Metabolism & Disposition
(Your Name) thought you would be interested in this article in Drug Metabolism & Disposition.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Research ArticleArticle

Nicotine Kinetics in Zebra Finches

Sharon Miksys, Susanne L.T. Cappendijk, William M. Perry, Bin Zhao and Rachel F. Tyndale
Drug Metabolism and Disposition June 1, 2013, 41 (6) 1240-1246; DOI: https://doi.org/10.1124/dmd.112.050765

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero

Share
Research ArticleArticle

Nicotine Kinetics in Zebra Finches

Sharon Miksys, Susanne L.T. Cappendijk, William M. Perry, Bin Zhao and Rachel F. Tyndale
Drug Metabolism and Disposition June 1, 2013, 41 (6) 1240-1246; DOI: https://doi.org/10.1124/dmd.112.050765
Reddit logo Twitter logo Facebook logo Mendeley logo
  • Tweet Widget
  • Facebook Like
  • Google Plus One

Jump to section

  • Article
    • Abstract
    • Introduction
    • Materials and Methods
    • Results
    • Discussion
    • Acknowledgments
    • Authorship Contributions
    • Footnotes
    • Abbreviations
    • References
  • Figures & Data
  • Info & Metrics
  • eLetters
  • PDF

Related Articles

Cited By...

More in this TOC Section

  • A PBPK model for CBD in adults and children
  • Olanzapine Glucuronidation in Humanized Mice
  • rs2242480 Regulates the Expression of CYP3A4 and CYP3A5
Show more Articles

Similar Articles

Advertisement
  • Home
  • Alerts
Facebook   Twitter   LinkedIn   RSS

Navigate

  • Current Issue
  • Fast Forward by date
  • Fast Forward by section
  • Latest Articles
  • Archive
  • Search for Articles
  • Feedback
  • ASPET

More Information

  • About DMD
  • Editorial Board
  • Instructions to Authors
  • Submit a Manuscript
  • Customized Alerts
  • RSS Feeds
  • Subscriptions
  • Permissions
  • Terms & Conditions of Use

ASPET's Other Journals

  • Journal of Pharmacology and Experimental Therapeutics
  • Molecular Pharmacology
  • Pharmacological Reviews
  • Pharmacology Research & Perspectives
ISSN 1521-009X (Online)

Copyright © 2023 by the American Society for Pharmacology and Experimental Therapeutics