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Research ArticleArticle

In Vitro Hydrolysis and Transesterification of CDP323, an α4β1/α4β7 Integrin Antagonist Ester Prodrug

Hugues Chanteux, Maria Rosa, Claude Delatour, Chandra Prakash, Steven Smith and Jean-Marie Nicolas
Drug Metabolism and Disposition January 2014, 42 (1) 153-161; DOI: https://doi.org/10.1124/dmd.113.054049
Hugues Chanteux
UCB Pharma SA, Non Clinical DMPK, Braine l’Alleud, Belgium (H.C., M.R., C.D., S.S., J.M.N.); Biogenidec, Cambridge, Massachusetts (C.P.)
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Maria Rosa
UCB Pharma SA, Non Clinical DMPK, Braine l’Alleud, Belgium (H.C., M.R., C.D., S.S., J.M.N.); Biogenidec, Cambridge, Massachusetts (C.P.)
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Claude Delatour
UCB Pharma SA, Non Clinical DMPK, Braine l’Alleud, Belgium (H.C., M.R., C.D., S.S., J.M.N.); Biogenidec, Cambridge, Massachusetts (C.P.)
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Chandra Prakash
UCB Pharma SA, Non Clinical DMPK, Braine l’Alleud, Belgium (H.C., M.R., C.D., S.S., J.M.N.); Biogenidec, Cambridge, Massachusetts (C.P.)
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Steven Smith
UCB Pharma SA, Non Clinical DMPK, Braine l’Alleud, Belgium (H.C., M.R., C.D., S.S., J.M.N.); Biogenidec, Cambridge, Massachusetts (C.P.)
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Jean-Marie Nicolas
UCB Pharma SA, Non Clinical DMPK, Braine l’Alleud, Belgium (H.C., M.R., C.D., S.S., J.M.N.); Biogenidec, Cambridge, Massachusetts (C.P.)
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Abstract

We identified the enzyme(s) involved in the hydrolysis of the ethyl ester prodrug CDP323 (C28H29BrN403) and characterized its transesterification in the presence of ethanol with special emphasis on the risks of drug-drug interaction. The hydrolysis of CDP323 was evaluated in vitro using human liver and intestinal microsomes and recombinant human carboxylesterases (hCES1 and 2) and was shown to be approximately 20-fold higher in human liver microsomes when compared with human intestinal microsomes and in hCES1 when compared with hCES2. Nonspecific inhibitors of carboxylesterases significantly inhibited the hydrolysis of CDP323 (>80% inhibition) while specific inhibitors of CES2, acetylcholine esterase, arylesterase, and butyrylcholinesterase did not impair the hydrolysis reaction. The effect of ethanol on the kinetic parameters for hydrolysis was investigated, demonstrating that at high concentration (2%), Michaelis-Menten constant (Km), maximum velocity (Vmax), and intrinsic clearance (CLint) for the formation of the hydrolyzed product were decreased (∼40%). The use of deuterated ethanol allowed more mechanistic investigations of the transesterification mechanism and showed that the intrinsic clearance based on parent loss was not impaired in the presence of alcohol. Overall, our data demonstrate that CDP323 is mainly hydrolyzed by hCES1 and is prone to transesterification in the presence of ethanol. Transesterification mechanisms compete with hydrolysis without impairing the overall clearance of the ester prodrug. Based on in vitro results, the risk of a clinically significant drug-drug interaction with ethanol is anticipated to be low.

Footnotes

    • Received August 7, 2013.
    • Accepted October 31, 2013.
  • dx.doi.org/10.1124/dmd.113.054049.

  • Copyright © 2013 by The American Society for Pharmacology and Experimental Therapeutics
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Drug Metabolism and Disposition: 42 (1)
Drug Metabolism and Disposition
Vol. 42, Issue 1
1 Jan 2014
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Research ArticleArticle

Hydrolysis and Transesterification of CDP323 Prodrug

Hugues Chanteux, Maria Rosa, Claude Delatour, Chandra Prakash, Steven Smith and Jean-Marie Nicolas
Drug Metabolism and Disposition January 1, 2014, 42 (1) 153-161; DOI: https://doi.org/10.1124/dmd.113.054049

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Research ArticleArticle

Hydrolysis and Transesterification of CDP323 Prodrug

Hugues Chanteux, Maria Rosa, Claude Delatour, Chandra Prakash, Steven Smith and Jean-Marie Nicolas
Drug Metabolism and Disposition January 1, 2014, 42 (1) 153-161; DOI: https://doi.org/10.1124/dmd.113.054049
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