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Research ArticlePerspective

Lost in Centrifugation: Accounting for Transporter Protein Losses in Quantitative Targeted Absolute Proteomics

Matthew D Harwood, Matthew R Russell, Sibylle Neuhoff, Geoffrey Warhurst and Amin Rostami-Hodjegan
Drug Metabolism and Disposition October 2014, 42 (10) 1766-1772; DOI: https://doi.org/10.1124/dmd.114.058446
Matthew D Harwood
Gut Barrier Group, Inflammation & Repair, University of Manchester, Salford Royal NHS Trust, Salford (M.D.H., G.W.), Simcyp Limited (a Certara Company), Blades Enterprise Centre, Sheffield, (M.D.H., S.N., A.R-H.), and Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, Manchester (M.R.R., A.R-H.), United Kingdom
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Matthew R Russell
Gut Barrier Group, Inflammation & Repair, University of Manchester, Salford Royal NHS Trust, Salford (M.D.H., G.W.), Simcyp Limited (a Certara Company), Blades Enterprise Centre, Sheffield, (M.D.H., S.N., A.R-H.), and Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, Manchester (M.R.R., A.R-H.), United Kingdom
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Sibylle Neuhoff
Gut Barrier Group, Inflammation & Repair, University of Manchester, Salford Royal NHS Trust, Salford (M.D.H., G.W.), Simcyp Limited (a Certara Company), Blades Enterprise Centre, Sheffield, (M.D.H., S.N., A.R-H.), and Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, Manchester (M.R.R., A.R-H.), United Kingdom
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Geoffrey Warhurst
Gut Barrier Group, Inflammation & Repair, University of Manchester, Salford Royal NHS Trust, Salford (M.D.H., G.W.), Simcyp Limited (a Certara Company), Blades Enterprise Centre, Sheffield, (M.D.H., S.N., A.R-H.), and Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, Manchester (M.R.R., A.R-H.), United Kingdom
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Amin Rostami-Hodjegan
Gut Barrier Group, Inflammation & Repair, University of Manchester, Salford Royal NHS Trust, Salford (M.D.H., G.W.), Simcyp Limited (a Certara Company), Blades Enterprise Centre, Sheffield, (M.D.H., S.N., A.R-H.), and Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, Manchester (M.R.R., A.R-H.), United Kingdom
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Article Information

vol. 42 no. 10 1766-1772
DOI 
https://doi.org/10.1124/dmd.114.058446
PubMed 
25061159

Published By 
American Society for Pharmacology and Experimental Therapeutics
Print ISSN 
0090-9556
Online ISSN 
1521-009X
History 
  • Received April 4, 2014
  • Accepted July 24, 2014
  • Published online September 5, 2014.

Article Versions

  • Earlier version (July 24, 2014 - 07:14).
  • You are viewing the most recent version of this article.
Copyright & Usage 
Copyright © 2014 by The American Society for Pharmacology and Experimental Therapeutics

Author Information

  1. Matthew D Harwood,
  2. Matthew R Russell,
  3. Sibylle Neuhoff,
  4. Geoffrey Warhurst, and
  5. Amin Rostami-Hodjegan
  1. Gut Barrier Group, Inflammation & Repair, University of Manchester, Salford Royal NHS Trust, Salford (M.D.H., G.W.), Simcyp Limited (a Certara Company), Blades Enterprise Centre, Sheffield, (M.D.H., S.N., A.R-H.), and Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, Manchester (M.R.R., A.R-H.), United Kingdom
  1. Address correspondence to:
    Matthew Harwood, Gut Barrier Group, Inflammation & Repair, University of Manchester, Salford Royal NHS Trust, Salford, M6 8HD, UK. E-mail: matthew.harwood{at}certara.com
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Drug Metabolism and Disposition: 42 (10)
Drug Metabolism and Disposition
Vol. 42, Issue 10
1 Oct 2014
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Research ArticlePerspective

Accounting for Protein Loss in Absolute Abundance Analysis

Matthew D Harwood, Matthew R Russell, Sibylle Neuhoff, Geoffrey Warhurst and Amin Rostami-Hodjegan
Drug Metabolism and Disposition October 1, 2014, 42 (10) 1766-1772; DOI: https://doi.org/10.1124/dmd.114.058446

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Research ArticlePerspective

Accounting for Protein Loss in Absolute Abundance Analysis

Matthew D Harwood, Matthew R Russell, Sibylle Neuhoff, Geoffrey Warhurst and Amin Rostami-Hodjegan
Drug Metabolism and Disposition October 1, 2014, 42 (10) 1766-1772; DOI: https://doi.org/10.1124/dmd.114.058446
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  • Article
    • Abstract
    • Introduction
    • Subcellular Fractionation in Membrane Proteomics
    • Protein Losses in Centrifugation: The Utility of Recovery Factors
    • Accounting for Protein Losses in QTAP Abundance Analysis
    • Correcting for Protein Losses in Centrifugation
    • Concluding Remarks
    • Acknowledgments
    • Authorship Contributions
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