Research ArticlePerspective
Lost in Centrifugation: Accounting for Transporter Protein Losses in Quantitative Targeted Absolute Proteomics
Matthew D Harwood, Matthew R Russell, Sibylle Neuhoff, Geoffrey Warhurst and Amin Rostami-Hodjegan
Drug Metabolism and Disposition October 2014, 42 (10) 1766-1772; DOI: https://doi.org/10.1124/dmd.114.058446
Matthew D Harwood
Gut Barrier Group, Inflammation & Repair, University of Manchester, Salford Royal NHS Trust, Salford (M.D.H., G.W.), Simcyp Limited (a Certara Company), Blades Enterprise Centre, Sheffield, (M.D.H., S.N., A.R-H.), and Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, Manchester (M.R.R., A.R-H.), United Kingdom
Matthew R Russell
Gut Barrier Group, Inflammation & Repair, University of Manchester, Salford Royal NHS Trust, Salford (M.D.H., G.W.), Simcyp Limited (a Certara Company), Blades Enterprise Centre, Sheffield, (M.D.H., S.N., A.R-H.), and Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, Manchester (M.R.R., A.R-H.), United Kingdom
Sibylle Neuhoff
Gut Barrier Group, Inflammation & Repair, University of Manchester, Salford Royal NHS Trust, Salford (M.D.H., G.W.), Simcyp Limited (a Certara Company), Blades Enterprise Centre, Sheffield, (M.D.H., S.N., A.R-H.), and Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, Manchester (M.R.R., A.R-H.), United Kingdom
Geoffrey Warhurst
Gut Barrier Group, Inflammation & Repair, University of Manchester, Salford Royal NHS Trust, Salford (M.D.H., G.W.), Simcyp Limited (a Certara Company), Blades Enterprise Centre, Sheffield, (M.D.H., S.N., A.R-H.), and Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, Manchester (M.R.R., A.R-H.), United Kingdom
Amin Rostami-Hodjegan
Gut Barrier Group, Inflammation & Repair, University of Manchester, Salford Royal NHS Trust, Salford (M.D.H., G.W.), Simcyp Limited (a Certara Company), Blades Enterprise Centre, Sheffield, (M.D.H., S.N., A.R-H.), and Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, Manchester (M.R.R., A.R-H.), United Kingdom
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Research ArticlePerspective
Accounting for Protein Loss in Absolute Abundance Analysis
Matthew D Harwood, Matthew R Russell, Sibylle Neuhoff, Geoffrey Warhurst and Amin Rostami-Hodjegan
Drug Metabolism and Disposition October 1, 2014, 42 (10) 1766-1772; DOI: https://doi.org/10.1124/dmd.114.058446
Jump to section
- Article
- Abstract
- Introduction
- Subcellular Fractionation in Membrane Proteomics
- Protein Losses in Centrifugation: The Utility of Recovery Factors
- Accounting for Protein Losses in QTAP Abundance Analysis
- Correcting for Protein Losses in Centrifugation
- Concluding Remarks
- Acknowledgments
- Authorship Contributions
- Footnotes
- Abbreviations
- References
- Figures & Data
- Info & Metrics
- eLetters
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