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Rapid CommunicationShort Communication

UDP-Glucuronosyltransferase 1a Enzymes Are Present and Active in the Mouse Blastocyst

Abby C. Collier, Yasuhiro Yamauchi, Brittany L.M. Sato, Luc R.A. Rougée and Monika A. Ward
Drug Metabolism and Disposition November 2014, 42 (11) 1921-1925; DOI: https://doi.org/10.1124/dmd.114.059766
Abby C. Collier
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, Canada (A.C.C.); Department of Tropical Medicine, Medical Microbiology, and Pharmacology (A.C.C., B.L.M.S., L.R.A.R.), and Institute for Biogenesis Research (Y.Y., M.A.W.), John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii; and Natural Sciences and Mathematics, Chaminade University of Honolulu, Honolulu, Hawaii (B.L.M.S.)
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Yasuhiro Yamauchi
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, Canada (A.C.C.); Department of Tropical Medicine, Medical Microbiology, and Pharmacology (A.C.C., B.L.M.S., L.R.A.R.), and Institute for Biogenesis Research (Y.Y., M.A.W.), John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii; and Natural Sciences and Mathematics, Chaminade University of Honolulu, Honolulu, Hawaii (B.L.M.S.)
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Brittany L.M. Sato
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, Canada (A.C.C.); Department of Tropical Medicine, Medical Microbiology, and Pharmacology (A.C.C., B.L.M.S., L.R.A.R.), and Institute for Biogenesis Research (Y.Y., M.A.W.), John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii; and Natural Sciences and Mathematics, Chaminade University of Honolulu, Honolulu, Hawaii (B.L.M.S.)
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Luc R.A. Rougée
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, Canada (A.C.C.); Department of Tropical Medicine, Medical Microbiology, and Pharmacology (A.C.C., B.L.M.S., L.R.A.R.), and Institute for Biogenesis Research (Y.Y., M.A.W.), John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii; and Natural Sciences and Mathematics, Chaminade University of Honolulu, Honolulu, Hawaii (B.L.M.S.)
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Monika A. Ward
Faculty of Pharmaceutical Sciences, University of British Columbia, Vancouver, British Columbia, Canada (A.C.C.); Department of Tropical Medicine, Medical Microbiology, and Pharmacology (A.C.C., B.L.M.S., L.R.A.R.), and Institute for Biogenesis Research (Y.Y., M.A.W.), John A. Burns School of Medicine, University of Hawaii, Honolulu, Hawaii; and Natural Sciences and Mathematics, Chaminade University of Honolulu, Honolulu, Hawaii (B.L.M.S.)
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Abstract

The UDP-glucuronosyltransferase (UGT) enzymes are critical for regulating nutrients, hormones, and endobiotics, as well as for detoxifying xenobiotics. Human and murine fetuses are known to express glucuronidation enzymes, but there are currently no data prior to implantation. Here we addressed this gap in knowledge and tested whether Ugt enzymes are already present in preimplantation-stage embryos. Blastocysts were obtained after in vitro fertilization with gametes from B6D2F1 hybrid mice and from embryo culture. Protein expression and localization were determined using pan-specific UGT1A and UGT2B, as well as anti-human isoform-specific antibodies. Immunofluorescence analysis showed that blastocysts expressed Ugt1a globally, in the cytoplasm and nuclei of all of the cells. Western blots demonstrated the presence of Ugt1a6 but not Ugt1a1, Ugt1a3, Ugt1a4, or Ugt1a9. The Ugt2b proteins were not detected by either assay. The level of Ugt activity in murine blastocysts was comparable with that of the adult human liver (per milligram of protein), but the activity of β-glucuronidase, an Ugt-partnering enzyme responsible for substrate regeneration, was lower. Altogether, these data confirm that Ugt1a proteins are present and active in preimplantation murine embryos and point to a potential role for these proteins in implantation and early embryonic and fetal development.

Footnotes

    • Received June 27, 2014.
    • Accepted September 8, 2014.
  • This research was supported by the National Institutes of Health National Institute on Minority Health and Health Disparities [Grant G12-MD007601], the National Institutes of Health National Institute of General Medical Sciences [Grant P20-GM103457], the National Institutes of Health Eunice Kennedy Shriver National Institute of Child Health and Human Development [Grant R01-HD072380], and the Hawaii Community Foundation. The funding bodies had no input into the study design, interpretation, or decision to publish.

  • dx.doi.org/10.1124/dmd.114.059766.

  • Copyright © 2014 by The American Society for Pharmacology and Experimental Therapeutics
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Drug Metabolism and Disposition: 42 (11)
Drug Metabolism and Disposition
Vol. 42, Issue 11
1 Nov 2014
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Rapid CommunicationShort Communication

UGT in Mouse Blastocysts

Abby C. Collier, Yasuhiro Yamauchi, Brittany L.M. Sato, Luc R.A. Rougée and Monika A. Ward
Drug Metabolism and Disposition November 1, 2014, 42 (11) 1921-1925; DOI: https://doi.org/10.1124/dmd.114.059766

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Rapid CommunicationShort Communication

UGT in Mouse Blastocysts

Abby C. Collier, Yasuhiro Yamauchi, Brittany L.M. Sato, Luc R.A. Rougée and Monika A. Ward
Drug Metabolism and Disposition November 1, 2014, 42 (11) 1921-1925; DOI: https://doi.org/10.1124/dmd.114.059766
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