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Research ArticleArticle

Atenolol Renal Secretion Is Mediated by Human Organic Cation Transporter 2 and Multidrug and Toxin Extrusion Proteins

Jia Yin, Haichuan Duan, Yoshiyuki Shirasaka, Bhagwat Prasad and Joanne Wang
Drug Metabolism and Disposition December 2015, 43 (12) 1872-1881; DOI: https://doi.org/10.1124/dmd.115.066175
Jia Yin
Department of Pharmaceutics, University of Washington, Seattle, Washington
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Haichuan Duan
Department of Pharmaceutics, University of Washington, Seattle, Washington
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Yoshiyuki Shirasaka
Department of Pharmaceutics, University of Washington, Seattle, Washington
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Bhagwat Prasad
Department of Pharmaceutics, University of Washington, Seattle, Washington
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Joanne Wang
Department of Pharmaceutics, University of Washington, Seattle, Washington
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Abstract

Atenolol is a β-blocker widely used in the treatment of hypertension. Atenolol is cleared predominantly by the kidney by both glomerular filtration and active secretion, but the molecular mechanisms involved in its renal secretion are unclear. Using a panel of human embryonic kidney cell lines stably expressing human organic cation transporter (hOCT) 1–3, human organic anion transporter (hOAT) 1, hOAT3, human multidrug and toxin extrusion protein (hMATE) 1, and hMATE2-K, we found that atenolol interacted with both organic cation and anion transporters. However, it is transported by hOCT1, hOCT2, hMATE1, and hMATE2-K, but not by hOCT3, hOAT1, and hOAT3. A detailed kinetic analysis coupled with absolute quantification of membrane transporter proteins by liquid chromatography–tandem mass spectrometry revealed that atenolol is an excellent substrate for the renal transporters hOCT2, hMATE1, and hMATE2-K. The Km values for hOCT2, hMATE1, and hMATE2-K are 280 ± 4, 32 ± 5, and 76 ± 14 μM, respectively, and the calculated turnover numbers are 2.76, 0.41, and 2.20 s−1, respectively. To demonstrate unidirectional transepithelial transport of atenolol, we developed and functionally validated a hOCT2/hMATE1 double-transfected Madin-Darby canine kidney cell culture model. Transwell studies showed that atenolol transport in the basal (B)-to-apical (A) direction is 27-fold higher than in the A-to-B direction, whereas its B-to-A/A-to-B transport ratio was only 2 in the vector-transfected control cells. The overall permeability of atenolol in the B-to-A direction in hOCT2/hMATE1 cells was 44-fold higher than in control cells. Together, our data support that atenolol tubular secretion is mediated through the hOCT2/hMATEs secretion pathway and suggest a significant role of organic cation transporters in the disposition of an important antihypertensive drug.

Footnotes

    • Received July 3, 2015.
    • Accepted September 14, 2015.
  • ↵1 Current affiliation: Department of Biopharmaceutics, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan.

  • This study was supported by the National Institutes of Health National Institute of General Medical Sciences [Grant R01 GM066233] and the National Center for Advancing Translational Sciences [Grant TL1 TR000422]. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

  • dx.doi.org/10.1124/dmd.115.066175.

  • ↵Embedded ImageThis article has supplemental material available at dmd.aspetjournals.org.

  • Copyright © 2015 by The American Society for Pharmacology and Experimental Therapeutics
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Drug Metabolism and Disposition: 43 (12)
Drug Metabolism and Disposition
Vol. 43, Issue 12
1 Dec 2015
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Research ArticleArticle

Atenolol Renal Secretion Involves hOCT2/hMATEs

Jia Yin, Haichuan Duan, Yoshiyuki Shirasaka, Bhagwat Prasad and Joanne Wang
Drug Metabolism and Disposition December 1, 2015, 43 (12) 1872-1881; DOI: https://doi.org/10.1124/dmd.115.066175

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Research ArticleArticle

Atenolol Renal Secretion Involves hOCT2/hMATEs

Jia Yin, Haichuan Duan, Yoshiyuki Shirasaka, Bhagwat Prasad and Joanne Wang
Drug Metabolism and Disposition December 1, 2015, 43 (12) 1872-1881; DOI: https://doi.org/10.1124/dmd.115.066175
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