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Rapid CommunicationShort Communication

Quantitative Transporter Proteomics by Liquid Chromatography with Tandem Mass Spectrometry: Addressing Methodologic Issues of Plasma Membrane Isolation and Expression-Activity Relationship

Vineet Kumar, Bhagwat Prasad, Gabriela Patilea, Anshul Gupta, Laurent Salphati, Raymond Evers, Cornelis E. C. A. Hop and Jashvant D. Unadkat
Drug Metabolism and Disposition February 2015, 43 (2) 284-288; DOI: https://doi.org/10.1124/dmd.114.061614
Vineet Kumar
Department of Pharmaceutics, University of Washington, Seattle, Washington (V.K., B.P., G.P., J.D.U.); Drug Metabolism and Pharmacokinetics, Infection Innovative Medicines Unit, AstraZeneca Pharmaceuticals LLP, Waltham, Massachusetts (A.G.); Pharmacokinetics, Pharmacodynamics and Drug Metabolism, Merck & Co., Kenilworth, New Jersey (R.E.); Drug Metabolism and Pharmacokinetics, Genentech, South San Francisco, Californai (L.S., C.E.C.A.H.)
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Bhagwat Prasad
Department of Pharmaceutics, University of Washington, Seattle, Washington (V.K., B.P., G.P., J.D.U.); Drug Metabolism and Pharmacokinetics, Infection Innovative Medicines Unit, AstraZeneca Pharmaceuticals LLP, Waltham, Massachusetts (A.G.); Pharmacokinetics, Pharmacodynamics and Drug Metabolism, Merck & Co., Kenilworth, New Jersey (R.E.); Drug Metabolism and Pharmacokinetics, Genentech, South San Francisco, Californai (L.S., C.E.C.A.H.)
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Gabriela Patilea
Department of Pharmaceutics, University of Washington, Seattle, Washington (V.K., B.P., G.P., J.D.U.); Drug Metabolism and Pharmacokinetics, Infection Innovative Medicines Unit, AstraZeneca Pharmaceuticals LLP, Waltham, Massachusetts (A.G.); Pharmacokinetics, Pharmacodynamics and Drug Metabolism, Merck & Co., Kenilworth, New Jersey (R.E.); Drug Metabolism and Pharmacokinetics, Genentech, South San Francisco, Californai (L.S., C.E.C.A.H.)
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Anshul Gupta
Department of Pharmaceutics, University of Washington, Seattle, Washington (V.K., B.P., G.P., J.D.U.); Drug Metabolism and Pharmacokinetics, Infection Innovative Medicines Unit, AstraZeneca Pharmaceuticals LLP, Waltham, Massachusetts (A.G.); Pharmacokinetics, Pharmacodynamics and Drug Metabolism, Merck & Co., Kenilworth, New Jersey (R.E.); Drug Metabolism and Pharmacokinetics, Genentech, South San Francisco, Californai (L.S., C.E.C.A.H.)
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Laurent Salphati
Department of Pharmaceutics, University of Washington, Seattle, Washington (V.K., B.P., G.P., J.D.U.); Drug Metabolism and Pharmacokinetics, Infection Innovative Medicines Unit, AstraZeneca Pharmaceuticals LLP, Waltham, Massachusetts (A.G.); Pharmacokinetics, Pharmacodynamics and Drug Metabolism, Merck & Co., Kenilworth, New Jersey (R.E.); Drug Metabolism and Pharmacokinetics, Genentech, South San Francisco, Californai (L.S., C.E.C.A.H.)
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Raymond Evers
Department of Pharmaceutics, University of Washington, Seattle, Washington (V.K., B.P., G.P., J.D.U.); Drug Metabolism and Pharmacokinetics, Infection Innovative Medicines Unit, AstraZeneca Pharmaceuticals LLP, Waltham, Massachusetts (A.G.); Pharmacokinetics, Pharmacodynamics and Drug Metabolism, Merck & Co., Kenilworth, New Jersey (R.E.); Drug Metabolism and Pharmacokinetics, Genentech, South San Francisco, Californai (L.S., C.E.C.A.H.)
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Cornelis E. C. A. Hop
Department of Pharmaceutics, University of Washington, Seattle, Washington (V.K., B.P., G.P., J.D.U.); Drug Metabolism and Pharmacokinetics, Infection Innovative Medicines Unit, AstraZeneca Pharmaceuticals LLP, Waltham, Massachusetts (A.G.); Pharmacokinetics, Pharmacodynamics and Drug Metabolism, Merck & Co., Kenilworth, New Jersey (R.E.); Drug Metabolism and Pharmacokinetics, Genentech, South San Francisco, Californai (L.S., C.E.C.A.H.)
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Jashvant D. Unadkat
Department of Pharmaceutics, University of Washington, Seattle, Washington (V.K., B.P., G.P., J.D.U.); Drug Metabolism and Pharmacokinetics, Infection Innovative Medicines Unit, AstraZeneca Pharmaceuticals LLP, Waltham, Massachusetts (A.G.); Pharmacokinetics, Pharmacodynamics and Drug Metabolism, Merck & Co., Kenilworth, New Jersey (R.E.); Drug Metabolism and Pharmacokinetics, Genentech, South San Francisco, Californai (L.S., C.E.C.A.H.)
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Article Information

vol. 43 no. 2 284-288
DOI 
https://doi.org/10.1124/dmd.114.061614
PubMed 
25488931

Published By 
American Society for Pharmacology and Experimental Therapeutics
Print ISSN 
0090-9556
Online ISSN 
1521-009X
History 
  • Received October 17, 2014
  • Accepted December 8, 2014
  • Published online December 31, 2014.

Article Versions

  • Earlier version (December 8, 2014 - 06:43).
  • You are viewing the most recent version of this article.
Copyright & Usage 
Copyright © 2014 by The American Society for Pharmacology and Experimental Therapeutics

Author Information

  1. Vineet Kumar,
  2. Bhagwat Prasad,
  3. Gabriela Patilea,
  4. Anshul Gupta,
  5. Laurent Salphati,
  6. Raymond Evers,
  7. Cornelis E. C. A. Hop, and
  8. Jashvant D. Unadkat
  1. Department of Pharmaceutics, University of Washington, Seattle, Washington (V.K., B.P., G.P., J.D.U.); Drug Metabolism and Pharmacokinetics, Infection Innovative Medicines Unit, AstraZeneca Pharmaceuticals LLP, Waltham, Massachusetts (A.G.); Pharmacokinetics, Pharmacodynamics and Drug Metabolism, Merck & Co., Kenilworth, New Jersey (R.E.); Drug Metabolism and Pharmacokinetics, Genentech, South San Francisco, Californai (L.S., C.E.C.A.H.)
  1. Address correspondence to:
    Jashvant D. Unadkat, Department of Pharmaceutics, University of Washington, Seattle, P.O. Box 357610, WA 98195. E-mail: jash{at}u.washington.edu
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Drug Metabolism and Disposition: 43 (2)
Drug Metabolism and Disposition
Vol. 43, Issue 2
1 Feb 2015
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Rapid CommunicationShort Communication

Transporter Expression-Activity Correlation

Vineet Kumar, Bhagwat Prasad, Gabriela Patilea, Anshul Gupta, Laurent Salphati, Raymond Evers, Cornelis E. C. A. Hop and Jashvant D. Unadkat
Drug Metabolism and Disposition February 1, 2015, 43 (2) 284-288; DOI: https://doi.org/10.1124/dmd.114.061614

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Rapid CommunicationShort Communication

Transporter Expression-Activity Correlation

Vineet Kumar, Bhagwat Prasad, Gabriela Patilea, Anshul Gupta, Laurent Salphati, Raymond Evers, Cornelis E. C. A. Hop and Jashvant D. Unadkat
Drug Metabolism and Disposition February 1, 2015, 43 (2) 284-288; DOI: https://doi.org/10.1124/dmd.114.061614
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