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Research ArticleArticle

Meta-Analysis of Expression of Hepatic Organic Anion–Transporting Polypeptide (OATP) Transporters in Cellular Systems Relative to Human Liver Tissue

Justine Badée, Brahim Achour, Amin Rostami-Hodjegan and Aleksandra Galetin
Drug Metabolism and Disposition April 2015, 43 (4) 424-432; DOI: https://doi.org/10.1124/dmd.114.062034
Justine Badée
Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, The University of Manchester, Manchester, United Kingdom (J.B., B.A., A.R-H., A.G.) and Simcyp Limited (a Certara Company), Sheffield, United Kingdom (A.R-H.)
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Brahim Achour
Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, The University of Manchester, Manchester, United Kingdom (J.B., B.A., A.R-H., A.G.) and Simcyp Limited (a Certara Company), Sheffield, United Kingdom (A.R-H.)
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Amin Rostami-Hodjegan
Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, The University of Manchester, Manchester, United Kingdom (J.B., B.A., A.R-H., A.G.) and Simcyp Limited (a Certara Company), Sheffield, United Kingdom (A.R-H.)
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Aleksandra Galetin
Centre for Applied Pharmacokinetic Research, Manchester Pharmacy School, The University of Manchester, Manchester, United Kingdom (J.B., B.A., A.R-H., A.G.) and Simcyp Limited (a Certara Company), Sheffield, United Kingdom (A.R-H.)
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Abstract

Organic anion–transporting polypeptide (OATP)1B1, OATP1B3, and OATP2B1 transporters play an important role in hepatic drug disposition. Recently, an increasing number of studies have reported proteomic expression data for OATP transporters. However, systematic analysis and understanding of the actual differences in OATP expression between liver tissue and commonly used cellular systems is lacking. In the current study, meta-analysis was performed to assess the protein expression of OATP transporters reported in hepatocytes relative to liver tissue and to identify any potential correlations in transporter expression levels in the same individual. OATP1B1 was identified as the most abundant uptake transporter at 5.9 ± 8.3, 5.8 ± 3.3, and 4.2 ± 1.7 fmol/μg protein in liver tissue, sandwich-cultured human hepatocytes (SCHH), and cryopreserved suspended hepatocytes, respectively. The rank order in average expression in liver tissue and cellular systems was OATP1B1 > OATP1B3 ≈ OATP2B1. Abundance levels of the OATP transporters investigated were not significantly different between liver and cellular systems, with the exception of OATP2B1 expression in SCHH relative to liver tissue. Analysis of OATP1B1, OATP1B3, and OATP2B1 liver expression data in the same individuals (n = 86) identified weak (OATP1B1-OATP2B1) to moderately (OATP1B3-OATP2B1) significant correlations. A significant weak correlation was noted between OATP1B1 abundance and age of human donors, whereas expression of the OATPs investigated was independent of sex. Implications of the current analysis on the in vitro–in vivo extrapolation of transporter-mediated drug disposition using physiologically based pharmacokinetic models are discussed.

Footnotes

    • Received November 7, 2014.
    • Accepted January 7, 2015.
  • The work was funded by a consortium of pharmaceutical companies within the Centre for Applied Pharmacokinetic Research at the University of Manchester. J.B. is a recipient of a Ph.D. studentship from Biotechnology and Biological Sciences Research Council [BB/K501256/1] and AstraZeneca.

  • dx.doi.org/10.1124/dmd.114.062034.

  • ↵Embedded ImageThis article has supplemental material available at dmd.aspetjournals.org.

  • Copyright © 2015 by The American Society for Pharmacology and Experimental Therapeutics
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Drug Metabolism and Disposition: 43 (4)
Drug Metabolism and Disposition
Vol. 43, Issue 4
1 Apr 2015
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Research ArticleArticle

OATP Abundance in Liver Tissue and Cellular Systems

Justine Badée, Brahim Achour, Amin Rostami-Hodjegan and Aleksandra Galetin
Drug Metabolism and Disposition April 1, 2015, 43 (4) 424-432; DOI: https://doi.org/10.1124/dmd.114.062034

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Research ArticleArticle

OATP Abundance in Liver Tissue and Cellular Systems

Justine Badée, Brahim Achour, Amin Rostami-Hodjegan and Aleksandra Galetin
Drug Metabolism and Disposition April 1, 2015, 43 (4) 424-432; DOI: https://doi.org/10.1124/dmd.114.062034
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