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Research ArticleArticle

An Automated High-Throughput Metabolic Stability Assay Using an Integrated High-Resolution Accurate Mass Method and Automated Data Analysis Software

Pranav Shah, Edward Kerns, Dac-Trung Nguyen, R. Scott Obach, Amy Q. Wang, Alexey Zakharov, John McKew, Anton Simeonov, Cornelis E. C. A. Hop and Xin Xu
Drug Metabolism and Disposition October 2016, 44 (10) 1653-1661; DOI: https://doi.org/10.1124/dmd.116.072017
Pranav Shah
Division of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville Maryland (P.S, E.K, D-T.N, A Q.W, A.Z, J.M, A.S, X.X.); Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer. Groton, Connecticut (R.S.O.); and Department of Drug Metabolism and Pharmacokinetics, Genentech Inc., South San Francisco, California (C.E.C.A.H)
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Edward Kerns
Division of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville Maryland (P.S, E.K, D-T.N, A Q.W, A.Z, J.M, A.S, X.X.); Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer. Groton, Connecticut (R.S.O.); and Department of Drug Metabolism and Pharmacokinetics, Genentech Inc., South San Francisco, California (C.E.C.A.H)
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Dac-Trung Nguyen
Division of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville Maryland (P.S, E.K, D-T.N, A Q.W, A.Z, J.M, A.S, X.X.); Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer. Groton, Connecticut (R.S.O.); and Department of Drug Metabolism and Pharmacokinetics, Genentech Inc., South San Francisco, California (C.E.C.A.H)
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R. Scott Obach
Division of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville Maryland (P.S, E.K, D-T.N, A Q.W, A.Z, J.M, A.S, X.X.); Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer. Groton, Connecticut (R.S.O.); and Department of Drug Metabolism and Pharmacokinetics, Genentech Inc., South San Francisco, California (C.E.C.A.H)
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Amy Q. Wang
Division of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville Maryland (P.S, E.K, D-T.N, A Q.W, A.Z, J.M, A.S, X.X.); Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer. Groton, Connecticut (R.S.O.); and Department of Drug Metabolism and Pharmacokinetics, Genentech Inc., South San Francisco, California (C.E.C.A.H)
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Alexey Zakharov
Division of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville Maryland (P.S, E.K, D-T.N, A Q.W, A.Z, J.M, A.S, X.X.); Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer. Groton, Connecticut (R.S.O.); and Department of Drug Metabolism and Pharmacokinetics, Genentech Inc., South San Francisco, California (C.E.C.A.H)
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John McKew
Division of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville Maryland (P.S, E.K, D-T.N, A Q.W, A.Z, J.M, A.S, X.X.); Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer. Groton, Connecticut (R.S.O.); and Department of Drug Metabolism and Pharmacokinetics, Genentech Inc., South San Francisco, California (C.E.C.A.H)
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Anton Simeonov
Division of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville Maryland (P.S, E.K, D-T.N, A Q.W, A.Z, J.M, A.S, X.X.); Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer. Groton, Connecticut (R.S.O.); and Department of Drug Metabolism and Pharmacokinetics, Genentech Inc., South San Francisco, California (C.E.C.A.H)
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Cornelis E. C. A. Hop
Division of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville Maryland (P.S, E.K, D-T.N, A Q.W, A.Z, J.M, A.S, X.X.); Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer. Groton, Connecticut (R.S.O.); and Department of Drug Metabolism and Pharmacokinetics, Genentech Inc., South San Francisco, California (C.E.C.A.H)
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Xin Xu
Division of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville Maryland (P.S, E.K, D-T.N, A Q.W, A.Z, J.M, A.S, X.X.); Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer. Groton, Connecticut (R.S.O.); and Department of Drug Metabolism and Pharmacokinetics, Genentech Inc., South San Francisco, California (C.E.C.A.H)
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Article Information

vol. 44 no. 10 1653-1661
DOI 
https://doi.org/10.1124/dmd.116.072017
PubMed 
27417180

Published By 
American Society for Pharmacology and Experimental Therapeutics
Print ISSN 
0090-9556
Online ISSN 
1521-009X
History 
  • Received June 8, 2016
  • Accepted July 13, 2016
  • Published online August 31, 2016.

Article Versions

  • Earlier version (July 14, 2016 - 07:35).
  • Earlier version (July 22, 2016 - 07:34).
  • You are viewing the most recent version of this article.
Copyright & Usage 
U.S. Government work not protected by U.S. copyright

Author Information

  1. Pranav Shah,
  2. Edward Kerns,
  3. Dac-Trung Nguyen,
  4. R. Scott Obach,
  5. Amy Q. Wang,
  6. Alexey Zakharov,
  7. John McKew,
  8. Anton Simeonov,
  9. Cornelis E. C. A. Hop, and
  10. Xin Xu
  1. Division of Preclinical Innovation, National Center for Advancing Translational Sciences, Rockville Maryland (P.S, E.K, D-T.N, A Q.W, A.Z, J.M, A.S, X.X.); Department of Pharmacokinetics, Dynamics and Metabolism, Pfizer. Groton, Connecticut (R.S.O.); and Department of Drug Metabolism and Pharmacokinetics, Genentech Inc., South San Francisco, California (C.E.C.A.H)
  1. Address correspondence to:
    Dr. Xin Xu, National Center for Advancing Translational Sciences, Division of Preclinical Innovation, 9800 Medical Center Dr., Rockville, MD 20850. E-mail: xin.xu3{at}nih.gov
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Drug Metabolism and Disposition: 44 (10)
Drug Metabolism and Disposition
Vol. 44, Issue 10
1 Oct 2016
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Research ArticleArticle

Automated High-Throughput Metabolic Stability Assay Method

Pranav Shah, Edward Kerns, Dac-Trung Nguyen, R. Scott Obach, Amy Q. Wang, Alexey Zakharov, John McKew, Anton Simeonov, Cornelis E. C. A. Hop and Xin Xu
Drug Metabolism and Disposition October 1, 2016, 44 (10) 1653-1661; DOI: https://doi.org/10.1124/dmd.116.072017

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Research ArticleArticle

Automated High-Throughput Metabolic Stability Assay Method

Pranav Shah, Edward Kerns, Dac-Trung Nguyen, R. Scott Obach, Amy Q. Wang, Alexey Zakharov, John McKew, Anton Simeonov, Cornelis E. C. A. Hop and Xin Xu
Drug Metabolism and Disposition October 1, 2016, 44 (10) 1653-1661; DOI: https://doi.org/10.1124/dmd.116.072017
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