Article Figures & Data
Figures
- Fig. 1.
ATP content evaluation in SCHH following 24- (A) and 72-hour exposure to varying concentrations of SSE and SCE. Aflatoxin (10 µM) was used as a positive control cytotoxic agent. *P value ≤0.05 when compared with solvent control. Data are from a single experiment with treatments performed in triplicate wells. Mean values are reported ± S.D.
- Fig. 2.
Inhibition of CYP3A4/5 by SSE (A) or SCE (B), without preincubation (●) and with preincubation (■), evaluated in SCHH by monitoring the formation of OHMDZ from DZ. SSE no preincubation IC50 = 1.23 µg/ml, 95% confidence interval = 0.823–1.85, goodness of fit = 0.989; SSE preincubation IC50 = 0.378 µg/ml, 95% confidence interval = 0.299–0.479, goodness of fit = 0.999. SCE no preincubation IC50 = 4.46 µg/ml, 95% confidence interval = 4.44–4.48, goodness of fit = 1.0; SCE preincubation IC50 = 1.45 µg/ml, 95% confidence interval = 1.44–1.46, goodness of fit = 1.0. Data are from a single experiment with treatments performed in triplicate wells. Mean values are reported ± S.D.
- Fig. 3.
Relative-fold change of CYP3A4 mRNA content in SCHH following 72 hours of treatment with RIF (10 µM), SJW (20 µg/ml), SSE, or SCE. Error bars represent 95% confidence intervals. Data are from a single experiment with treatments performed in triplicate wells.
- Fig. 4.
Intracellular concentrations of schizandrin, gomisin A, and schisandrin A in SCHH following 72 hours of treatment with SSE or SCE. Data from a single experiment with treatments performed in triplicate wells. Mean values reported ± S.D.
- Fig. 5.
SCHH were treated with RIF, SJW, SSE, or SCE for 72 hours prior to an MDZ (1 µM) incubation of 24 hours, monitoring for depletion of substrate. Disappearance of MDZ from the SCHH system was plotted over time. Data are from a single experiment with treatments performed in triplicate wells. Mean values are reported ± S.D.
- Fig. 6.
Intrinsic clearance of MDZ was calculated from linear regression analysis of the percentage of parent remaining (log transformed) versus time profile to estimate the elimination rate. MDZ intrinsic clearance in SCHH treated with SJW (A), RIF (A), SSE (B), or SCE (B) for 72 hour. *P value ≤0.05 when compared with solvent control. Data are from a single experiment with treatments performed in triplicate wells. Mean values reported ± S.D.
Tables
Hepatocyte Lot Sex Experiment Figure HUM4059 Female 24-hour cytotoxicity assessment Fig. 1A HUM4075B Male 72-hour cytotoxicity assessment Fig. 1B HUM4059 Female CYP3A4/5 inhibition evaluation Fig. 2 HUM4109 Female CYP3A4 mRNA induction assessment Fig. 3 HUM4122C Male Intracellular concentration evaluation Fig. 4 HUM4122C Male MDZ intrinsic clearance studies Figs. 5 and 6
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In Vitro Prediction of Clinical Herb-Drug Interactions
