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Research ArticleArticle

Development and Validation of a Higher-Throughput Cytochrome P450 Inhibition Assay with the Novel Cofactor-Supplemented Permeabilized Cryopreserved Human Hepatocytes (MetMax Human Hepatocytes)

Veera Raghava Choudary Palacharla, Prathyusha Chunduru, Devender Reddy Ajjala, Gopinadh Bhyrapuneni, Ramakrishna Nirogi and Albert P. Li
Drug Metabolism and Disposition October 2019, 47 (10) 1032-1039; DOI: https://doi.org/10.1124/dmd.119.088237
Veera Raghava Choudary Palacharla
Drug Metabolism and Pharmacokinetics, Suven Life Sciences Ltd., Jeedimetla, Hyderabad, India (V.R.C.P., G.B., R.N.); Bio-analysis, Suven Life Sciences Ltd., Pashamylaram, Medak, India (P.C., D.R.A.); and In Vitro ADMET Laboratories Inc., Columbia, Maryland (A.P.L.)
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  • ORCID record for Veera Raghava Choudary Palacharla
Prathyusha Chunduru
Drug Metabolism and Pharmacokinetics, Suven Life Sciences Ltd., Jeedimetla, Hyderabad, India (V.R.C.P., G.B., R.N.); Bio-analysis, Suven Life Sciences Ltd., Pashamylaram, Medak, India (P.C., D.R.A.); and In Vitro ADMET Laboratories Inc., Columbia, Maryland (A.P.L.)
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Devender Reddy Ajjala
Drug Metabolism and Pharmacokinetics, Suven Life Sciences Ltd., Jeedimetla, Hyderabad, India (V.R.C.P., G.B., R.N.); Bio-analysis, Suven Life Sciences Ltd., Pashamylaram, Medak, India (P.C., D.R.A.); and In Vitro ADMET Laboratories Inc., Columbia, Maryland (A.P.L.)
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Gopinadh Bhyrapuneni
Drug Metabolism and Pharmacokinetics, Suven Life Sciences Ltd., Jeedimetla, Hyderabad, India (V.R.C.P., G.B., R.N.); Bio-analysis, Suven Life Sciences Ltd., Pashamylaram, Medak, India (P.C., D.R.A.); and In Vitro ADMET Laboratories Inc., Columbia, Maryland (A.P.L.)
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Ramakrishna Nirogi
Drug Metabolism and Pharmacokinetics, Suven Life Sciences Ltd., Jeedimetla, Hyderabad, India (V.R.C.P., G.B., R.N.); Bio-analysis, Suven Life Sciences Ltd., Pashamylaram, Medak, India (P.C., D.R.A.); and In Vitro ADMET Laboratories Inc., Columbia, Maryland (A.P.L.)
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  • For correspondence: nvsrk@suven.com
Albert P. Li
Drug Metabolism and Pharmacokinetics, Suven Life Sciences Ltd., Jeedimetla, Hyderabad, India (V.R.C.P., G.B., R.N.); Bio-analysis, Suven Life Sciences Ltd., Pashamylaram, Medak, India (P.C., D.R.A.); and In Vitro ADMET Laboratories Inc., Columbia, Maryland (A.P.L.)
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  • ORCID record for Albert P. Li
  • For correspondence: lialbert@invitroadmet.com
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Article Information

vol. 47 no. 10 1032-1039
DOI 
https://doi.org/10.1124/dmd.119.088237
PubMed 
31375472

Published By 
American Society for Pharmacology and Experimental Therapeutics
Print ISSN 
0090-9556
Online ISSN 
1521-009X
History 
  • Received June 6, 2019
  • Accepted July 22, 2019
  • Published online September 13, 2019.

Article Versions

  • Earlier version (August 2, 2019 - 13:02).
  • You are viewing the most recent version of this article.
Copyright & Usage 
Copyright © 2019 by The American Society for Pharmacology and Experimental Therapeutics

Author Information

  1. Veera Raghava Choudary Palacharla,
  2. Prathyusha Chunduru,
  3. Devender Reddy Ajjala,
  4. Gopinadh Bhyrapuneni,
  5. Ramakrishna Nirogi⇑, and
  6. Albert P. Li⇑
  1. Drug Metabolism and Pharmacokinetics, Suven Life Sciences Ltd., Jeedimetla, Hyderabad, India (V.R.C.P., G.B., R.N.); Bio-analysis, Suven Life Sciences Ltd., Pashamylaram, Medak, India (P.C., D.R.A.); and In Vitro ADMET Laboratories Inc., Columbia, Maryland (A.P.L.)
  1. Address correspondence to:
    Dr. Ramakrishna Nirogi, Discovery Research, Suven Life Sciences Ltd, Serene Chambers, Road # 7, Banjara Hills, Hyderabad 500034, India. E-mail: nvsrk{at}suven.com; or
  2. Dr. Albert P. Li, In Vitro ADMET Laboratories Inc., 9221 Rumsey Road Suite 8, Columbia, MD 21045. E-mail: lialbert{at}invitroadmet.com
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Drug Metabolism and Disposition: 47 (10)
Drug Metabolism and Disposition
Vol. 47, Issue 10
1 Oct 2019
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Research ArticleArticle

High-Throughput P450 Inhibition Assay with MetMax Hepatocytes

Veera Raghava Choudary Palacharla, Prathyusha Chunduru, Devender Reddy Ajjala, Gopinadh Bhyrapuneni, Ramakrishna Nirogi and Albert P. Li
Drug Metabolism and Disposition October 1, 2019, 47 (10) 1032-1039; DOI: https://doi.org/10.1124/dmd.119.088237

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Research ArticleArticle

High-Throughput P450 Inhibition Assay with MetMax Hepatocytes

Veera Raghava Choudary Palacharla, Prathyusha Chunduru, Devender Reddy Ajjala, Gopinadh Bhyrapuneni, Ramakrishna Nirogi and Albert P. Li
Drug Metabolism and Disposition October 1, 2019, 47 (10) 1032-1039; DOI: https://doi.org/10.1124/dmd.119.088237
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