Article Figures & Data
Figures
Tables
Data Supplement
- Supplemental Data -
Supplementary Table 1 - Accurate mass measurement of monooxygenated PUFAs formed in the presence of human P450 2S1.
Supplementary Table 2 - P450 RNA abundance in gastrointestinal tract.
Supplementary Figure 1- Extracted ion chromatogram of m/z 319.2279 of rat tissue extract.
Supplementary Figure 2 - HRMS full scan of rat tissue extracts incubated with P450 2S1 in the presence of 16O2, 16O2/18O2, and 18O2.
Supplementary Figure 3 - Extracted ion chromatograms (m/z 319.2279) and HRMS (ESI-) mass fragmentation spectra from the incubation of arachidonic acid with human P450 2S1 and an NADPH-generating system.
Supplementary Figure 4 - Chromatogram showing complete derivatization of linoleic acid into its corresponding PFB ester.
Supplementary Figure 5 - HRMS (ESI-) mass fragmentation spectrum of -1 hydroxylinoleic acid formed by incubation of linoleic acid with human P450 2S1 and an NADPH-generating system.
Supplementary Figure 6 - Steady-state kinetic analysis of (-1)-hydroxylated fatty acids by P450 2S1.
- Supplemental Data -